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为研究抗CD4 McAb引起胸腺细胞减少的机理,本课题探讨了CD4 McAb诱导胸腺细胞发生凋亡(Apoptosis)的可能性。小鼠注射抗CD4 McAb后,皮质胸腺细胞体积缩小,染色质凝聚;PI染色后经流式细胞计测定,显示大量的亚二倍体细胞(凋亡细胞,Apoptotic cells),与正常小鼠相比P<0.001;片断化DNA的百分比也明显高于正常小鼠,P<0.01。片断化DNA在凝胶电泳上呈现典型的阶梯现象。细胞内钙离子浓度升高。放线菌酮对抗CD4McAb诱导的DNA片断化有一定的抑制作用。上述结果提示抗CD4 McAb促进胸腺细胞凋亡可能是胸腺细胞减少的原因之一。
In order to study the mechanism of anti-CD4 McAb-induced thymocyte reduction, we investigated the possibility of CD4 McAb-induced Apoptosis. After mice were injected with anti-CD4 McAb, cortical thymocytes were reduced in size and chromatin was aggregated. After PI staining, flow cytometry showed that a large number of sub-diploid cells (Apoptotic cells) P <0.001; the percentage of fragmented DNA was also significantly higher than that of normal mice (P <0.01). Fragmentation of DNA in gel electrophoresis showed a typical ladder phenomenon. Increased intracellular calcium concentration. Cycloheximide against CD4McAb-induced DNA fragmentation has a certain degree of inhibition. These results suggest that anti-CD4 McAb thymocyte apoptosis may be one of the causes of thymus reduction.