论文部分内容阅读
目的:观察损伤血管细胞周期素依赖激酶抑制蛋白P27的表达情况,探讨益气活血方对球囊损伤大鼠颈总动脉内膜平滑肌细胞增殖的干预机制。方法:随机将60只雄性SD大鼠分为模型组,阿司匹林组,益气活血方大、中、小剂量组。球囊损伤大鼠颈总动脉前给予高脂饲料喂养30天,造成高脂血症;然后经右股动脉穿刺球囊扩张损伤大鼠左颈总动脉,造成颈总动脉管腔的狭窄。手术前1天开始给药,每组动物于术后(14、28天)处死,取损伤颈总动脉,常规固定、包埋、切片,用免疫组织化学SABC法检测P27的表达。结果:各组P27均有表达,益气活血方大剂量组表达明显增多,用益气活血方干预后14天和28天P27表达量与模型组比较有显著性差异(P<0.05)。结论:益气活血方对球囊损伤后动脉血管内膜VSMCs增生有明显的抑制作用,其机制可能与上调P27的表达,抑制血管内膜VSMCs的细胞分裂周期进程有关。
OBJECTIVE: To observe the expression of cyclin-dependent kinase inhibitor protein P27 in injured blood vessels and to explore the intervention mechanism of Yiqi Huoxue recipe on the proliferation of rat carotid artery intimal smooth muscle cells. Methods: Sixty male Sprague-Dawley rats were randomly divided into model group, aspirin group, and large, medium and small doses of Yiqi Huoxue Fang. The balloon-injured rat carotid arteries were fed with high-fat diet for 30 days to cause hyperlipidemia. Then the right common carotid artery was punctured by balloon dilatation to damage the left common carotid artery, resulting in narrowing of the common carotid artery lumen. The administration was started 1 day before surgery. Each group of animals was sacrificed on the 14th and 28th days after operation. The common carotid artery was injured and routinely fixed, embedded and sectioned. The expression of P27 was detected by immunohistochemical SABC method. Results: The expression of P27 in all groups was increased, and the expression of Yiqi Huoxue Recipe was significantly increased. The expression of P27 at 14 and 28 days after intervention with Yiqi Huoxue Recipe was significantly different from that of the model group (P<0.05). Conclusion: Yiqi Huoxue Decoction can obviously inhibit the proliferation of arterial endothelium VSMCs after balloon injury. The mechanism may be related to up-regulating the expression of P27 and inhibiting the process of cell division cycle of VSMCs.