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建立了毛细管电泳电化学分离检测田基黄中生物活性成分芦丁和槲皮素的方法。考察了检测电极电位、缓冲液浓度、pH、运行电压和进样时间对分离的影响。以40 cm长,50μm内径的石英毛细管作为分离通道,运行缓冲液为25 mmol/L硼砂(pH 9.2)溶液,分离电压12 kV,0.3 mm直径的铂圆盘电极为检测电极,检测电位1.00 V(vs.Ag/AgCl),芦丁和槲皮素在10 min内得到良好分离。在上述实验条件下,芦丁和槲皮素分别在8.2×10-6~5.2×10-4mol/L与6.8×10-6~7.2×10-4mol/L范围内与峰面积呈良好线性关系,检出限分别为9.0×10-7mol/L(S/N=3)和4.7×10-7mol/L(S/N=3)。方法已应用于田基黄药材提取物成分分析。
A capillary electrophoresis method for the electrochemical separation of rutin and quercetin in bioactive compounds from Tianjihuang was established. The effects of electrode potential, buffer concentration, pH, operating voltage and injection time on the separation were investigated. A 40 cm long and 50 μm ID quartz capillary tube was used as the separation channel. The running buffer was 25 mmol / L borax (pH 9.2) solution. A platinum disk electrode with a voltage of 12 kV and a diameter of 0.3 mm was used as the detection electrode with a detection potential of 1.00 V (vs.Ag/AgCl), rutin and quercetin were well separated within 10 min. Under the above experimental conditions, rutin and quercetin showed a good linear relationship with the peak area in the ranges of 8.2 × 10-6 ~ 5.2 × 10-4mol / L and 6.8 × 10-6 ~ 7.2 × 10-4mol / L, respectively The detection limits were 9.0 × 10-7mol / L (S / N = 3) and 4.7 × 10-7mol / L (S / N = 3) respectively. The method has been applied to the constituents analysis of Radix et Rhizoma.