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目的观察肉苁蓉苯乙醇苷类成分(CPh Gs)对牛血清白蛋白(BSA)致大鼠肝纤维化的防治作用。方法将SD大鼠随机分为6组,每组12只,即正常对照组、肝纤维化模型组(模型组)、复方鳖甲软肝片阳性对照组(600 mg/kg)及CPh Gs高剂量组(500 mg/kg)、中剂量组(250 mg/kg)、低剂量组(125 mg/kg);采用静脉注射BSA诱导建立大鼠肝纤维化模型;造模同时,灌胃给予大鼠CPh Gs提取物,连续12 w,末次给药后腹主动脉采血,取出肝脏和脾脏。计算肝脾指数,检测大鼠血清肝功能指标及肝脏病理组织学变化。结果与正常对照组比较,模型组大鼠肝脏指数明显增加,血清AST含量明显增加。与模型组比较,复方鳖甲软肝片阳性对照组及CPh Gs不同剂量组大鼠肝脏指数均显著降低,CPh Gs不同剂量组大鼠脾脏指数明显下降,CPh Gs不同剂量组大鼠血清ALT、AST含量显著下降。CPh Gs可使BSA诱导的大鼠肝脏纤维增生明显减轻。病理结果显示,正常对照组大鼠肝组织未见异常,CPh Gs不同剂量组治疗效果显著,形态基本上接近于正常对照组。结论肉苁蓉苯乙醇苷类成分具有保护肝细胞和防治肝纤维化的作用。
Objective To investigate the preventive and therapeutic effects of CPh Gs on liver fibrosis induced by bovine serum albumin (BSA) in rats. Methods SD rats were randomly divided into 6 groups with 12 rats in each group, namely normal control group, liver fibrosis model group, Fufang Biejia Ruanganpian positive control group (600 mg / kg) and CPh Gs high (500 mg / kg), middle dose (250 mg / kg) and low dose (125 mg / kg). The rat model of hepatic fibrosis induced by intravenous injection of BSA was established. At the same time, Rat CPh Gs extract, continuous 12 w, after the last administration of abdominal aorta blood, remove the liver and spleen. The index of liver and spleen was calculated, and the indexes of serum liver function and histopathological changes of liver were detected. Results Compared with the normal control group, the liver index of the model group increased significantly and the serum AST level increased significantly. Compared with the model group, the liver index of Fufang Biejia Ruangan Tablet positive control group and CPh Gs different dose group were significantly decreased, while the CPh Gs different dose group rats spleen index decreased significantly, CPh Gs different doses of serum ALT, AST content decreased significantly. CPh Gs significantly attenuated BSA-induced hepatic fibrosis in rats. The pathological results showed that there was no abnormality in the liver of the normal control group, and the CPh Gs different dose group had a significant therapeutic effect. The morphology was basically close to that of the normal control group. Conclusion Cistanche phenylethanoid glycosides have the function of protecting hepatocytes and preventing and treating hepatic fibrosis.