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建立了测定血浆中左亚叶酸钙含量的RPHPLC方法。采用DiamonsilC18色谱柱(150mm×4.6mm,5μm),乙腈50mmol/L磷酸氢二钾/磷酸二氢钾缓冲溶液(10∶90,V/V,用磷酸调pH至4.0)为流动相,用前经0.45μm的过滤膜,超声脱气,流速为1.0mL/min,紫外检测波长为290nm,柱温为35℃,进样量20μL。该方法最低检出限为0.2ng;线性范围为0.05～10mg/L,方法平均回收率在97.0%以上;日内及日间精密度的相对标准偏差分别小于4%和6%。该方法简单,快速,灵敏度高,重复性好。
A RPHPLC method for the determination of levofloxacin in plasma was established. The mobile phase consisted of Diamonsil C18 column (150 mm × 4.6 mm, 5 μm), acetonitrile 50 mmol / L dipotassium phosphate / potassium dihydrogen phosphate buffer (10:90, V / V, adjusted to pH 4.0 with phosphoric acid) After 0.45μm filter membrane, ultrasonic degassing, flow rate of 1.0mL / min, UV detection wavelength of 290nm, the column temperature was 35 ℃, the injection volume of 20μL. The method has the lowest detection limit of 0.2ng, the linear range of 0.05 ~ 10mg / L and the average recovery of the method above 97.0%. The relative standard deviations (RSDs) of intra- and inter-day precision are less than 4% and 6% respectively. The method is simple, fast, high sensitivity and good repeatability.