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目的 观察戊四氮诱导癫痫发作的形式变化及不同温度下癫痫大鼠海马组织病理学的变化和计数CA3 区残存的神经元数量。方法 雄性SD大鼠分为上述不同温度的四组 ,用冰袋降温、白炽灯泡升温的方法来控制温度。将控制好温度的大鼠用戊四氮诱导癫痫发作 ,在相同的时间内观察大鼠发作形式的变化。HE染色后观察海马区组织病理学的改变 ,并选择组织学损害最明显的区域在高倍镜 ( 4 0× 10 )下计数神经元的丢失。结果 高温状态下癫痫发作程度最重 ,神经元丢失最严重 ,低温下癫痫发作程度较轻 ,神经元丢失也较少 ,亚低温下癫痫发作程度最轻 ,持续时间也最短 ,神经元丢失最少。结论 亚低温对癫痫大鼠有脑保护作用
Objective To observe the changes of pentylenetetrazole-induced seizures and the changes of hippocampal histopathology in epileptic rats at different temperatures and count the number of neurons remaining in the CA3 area. Methods Male Sprague-Dawley rats were divided into four groups with different temperatures mentioned above. The temperature was controlled by means of cooling with ice packs and heating of incandescent bulbs. Controlled rats were induced with pentylenetetrazol seizures, at the same time to observe the changes in rat seizures. The changes of histopathology in hippocampus were observed after HE staining, and the most obvious area of histological damage was selected to count the loss of neurons at high magnification (40 × 10). Results The highest degree of seizures occurred under high temperature and the neurons lost most seriously. The degree of seizures was lower at low temperature and the loss of neurons was also less. The seizure degree was the lightest and the duration was the lowest in mild hypothermia with the least loss of neurons. Conclusion Mild hypothermia has neuroprotective effects on epileptic rats