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目的:采用高效液相色谱法测定复方芬苯达唑片中芬苯达唑和吡喹酮的含量。方法:色谱柱:Inert Sustain~C18柱(250 mm×4.6 mm,5μm),流动相:乙腈-磷酸二氢钾缓冲液(p H=3)(48∶52),流速:1.0 ml·min~(~(-1)),检测波长:214 nm,柱温:30℃,进样量:10μl。结果:芬苯达唑和吡喹酮的线性范围分别为4.545~63.630μg·ml~(~(-1))(r=0.999 9)和0.225~3.150μg·ml~(~(-1))(r=0.999 9),平均回收率分别为101.09%(RSD=0.28%,n=9)和99.47%(RSD=0.78%,n=9)。结论:该方法简便、快捷、准确度高,适用于复方芬苯达唑片中芬苯达唑和吡喹酮的含量测定。
Objective: To determine the content of fenbendazole and praziquantel in compound fenbendazole tablets by high performance liquid chromatography. Methods: Column: Inert Sustain ~ C18 column (250 mm × 4.6 mm, 5 μm) with mobile phase of acetonitrile-potassium dihydrogen phosphate buffer (p H = 3) ~ (-1)), detection wavelength: 214 nm, column temperature: 30 ℃, injection volume: 10μl. Results: The linear range of fenbendazole and praziquantel was 4.545 ~ 63.630μg · ml ~ (-1) (r = 0.999 9) and 0.225 ~ 3.150μg · ml ~ (-1) respectively (r = 0.999 9). The average recoveries were 101.09% (RSD = 0.28%, n = 9) and 99.47% (RSD = 0.78%, n = 9) respectively. Conclusion: The method is simple, rapid, accurate and suitable for the determination of fenbendazole and praziquantel in compound fenbendazole tablets.