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优化重组大肠杆菌高密度发酵的调控工艺。利用重组大肠杆菌高密度发酵生产类人胶原蛋白,考察了连续流加和分阶段式两种不同补氮策略对细胞生长和类人胶原蛋白产量的影响。不同补氮方式显著影响类人胶原蛋白的产量。分阶段式补氮方式优于连续流加补氮方式,有利于细胞生长和类人胶原蛋白的表达,最终细胞浓度为76.01 g.L-1(DCW),类人胶原蛋白浓度为16.75 g.L-1,蛋白表达量为22.1%。
Optimization of recombinant Escherichia coli high-density fermentation process. The recombinant human Escherichia coli fermented with high density was used to produce human collagen, and the effect of two different nitrogen supplementation strategies on cell growth and humanoid collagen production was investigated. Different modes of nitrogen supplementation significantly affect the production of humanoid collagen. Staged nitrogen supplementation was superior to continuous nitrogen supplementation, which was conducive to cell growth and humanoid collagen expression. The final cell concentration was 76.01 gL-1 (DCW), the concentration of human-like collagen was 16.75 gL-1, The protein expression was 22.1%.