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目的:研究P27基因在卵巢上皮性恶性肿瘤中的表达及其临床意义。方法:用免疫组织化学方法检测18例卵巢正常组织、20例卵巢良性肿瘤及38例卵巢癌组织中P27基因的表达情况,并与临床特征相比较。结果:P27蛋白在卵巢上皮性恶、良性肿瘤和正常组织中阳性表达率分别为32%、65%和78%,P27蛋白在卵巢上皮性恶性肿瘤中的表达明显低于卵巢正常组织和卵巢良性肿瘤。在细胞分化程度I级的肿瘤中,P27蛋白表达阳性率明显高于分化程度Ⅱ级和Ⅲ级的肿瘤(P<0.01);在临床分期早期的肿瘤中,P27蛋白的表达率明显高于临床分期晚期的肿瘤(P<0.01)。不伴淋巴转移的P27蛋白的表达率明显高于有淋巴转移的蛋白的表达率(P<0.01)。结论:P27基因的表达与卵巢上皮性恶性肿瘤的细胞分化程度、临床分期及有无淋巴转移密切相关。临床检测卵巢上皮性肿瘤P27蛋白的表达,可以作为判断卵巢上皮性恶性肿瘤生物学行为及患者预后的参考指标。
Objective: To study the expression of P27 gene in epithelial ovarian cancer and its clinical significance. Methods: Immunohistochemistry was used to detect the expression of P27 gene in 18 cases of normal ovarian tissues, 20 cases of benign ovarian tumors and 38 cases of ovarian cancer, and compared with clinical features. Results: The positive rate of P27 protein was 32%, 65%, 78% respectively in ovarian epithelial malignant tumors, benign tumors and normal tissues. The expression of P27 protein in ovarian epithelial malignant tumors was significantly lower than that in normal ovarian tissues and benign ovarian tissues Tumor. The positive rate of P27 protein expression in grade I tumor was significantly higher than that in grade II and III tumors (P <0.01). The expression rate of P27 protein was significantly higher in clinical stage than in clinical stage Staged tumors (P <0.01). The expression rate of P27 protein without lymph node metastasis was significantly higher than that with lymph node metastasis (P <0.01). Conclusion: The expression of P27 gene is closely related to the degree of cell differentiation, clinical stage and lymphatic metastasis in ovarian epithelial malignant tumor. Clinical detection of epithelial ovarian cancer P27 protein expression can be used as a reference to determine the biological behavior of ovarian epithelial malignancies and prognosis of patients.