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Employing techniques of the affinity chromatography coveredwith ConA beads and protein ultrafiltration, certain proteins wereisolated and concentrated from the heated human seminal plasma.The results of computer assistant semen analyses (CASA) revealedthat the forward motility index of spermatozoa from bovine epididy-mal caput incubated with proteins and theophylline was significantlyhigher than that incubated with theophylline only (P < 0.01). Us-ing the methods of PAGE, SDS-PAGE and Coomassie blue stain-ing, the protein bands with high molecules in the gel of PAGEchanged into low molecules in the gel of SDS-PAGE. The majorbands of the proteins in the gel of SDS-PAGE were 46.8 and 57.5kDa. The proteins related to the forward motility of spermatozoa inmammalian seminal plasma play vital roles during sperm maturationin the epididymis and fertilization. (Chin J Androl 2001; 2: 87-91)
Employing techniques of the affinity chromatography covered with ConA beads and protein ultrafiltration, certain proteins wereisolated and concentrated from the heated human seminal plasma. The results of computer assistant semen analyzes (CASA) revealed that the forward motility index of spermatozoa from bovine epididy-mal caput incubated with proteins and theophylline was significantlyhigher than that incubated with theophylline only (P <0.01). Us-ing the methods of PAGE, SDS-PAGE and Coomassie blue stain-ing, the protein bands with high molecules in the gel of PAGEchanged into low molecules in The gel of SDS-PAGE. The major bands of the proteins in the gel of SDS-PAGE were 46.8 and 57.5 kDa. The proteins related to the forward motility of spermatozoa in mammalian seminal plasma play vital roles during sperm maturation in the epididymis and fertilization. (Chin J Androl 2001; 2: 87-91)