The present study provides a comparative understanding of florfenicol deposition kinetics following oral administration at a single dose of 30 mg / kg body weight to Japanese eel( Anguilla japonica) at the temperature of 20℃,24℃ and 28℃,respectively. Approximate 0. 3 m L of blood sample from each eel in these three groups was collected in a row at the time 0. 5,1,2,4,8,12,24 and 36 h after medicated-feed gavage. The concentrations of florfenicol in eel plasma were detected by reversed-phase high performance liquid chromatography( RP- HPLC). The plasma concentration-time data of each eel were analyzed by non-compartmental methods based on statistical moment theory using DAS software. Pharmacokinetics parameters of different groups were tested by one-way analysis of variance using SPSS software. It was found that most of parameters were different significantly among groups( P < 0. 05). In the three groups at 20℃,24℃ and 28℃,pharmacokinetics parameters of peak plasma concentration( Cmax) were( 7. 839 ± 1. 125),( 13. 010 ± 2. 334) and( 18. 267 ± 3. 717) μg/m L,and the time to reach the Cmax( Tmax) were( 6. 500 ± 2. 070),( 4. 500 ± 1. 414) and( 3. 429 ± 0. 926) h,respectively. These suggested that eels at higher temperature absorbed more drug and more quickly. The volumes of distribution( Vz/ F) were( 3. 964 ± 0. 594),( 2. 466 ± 0. 672) and( 1. 841 ± 0. 485) L / kg,respectively.The difference deduced that more florfenicol was bound to tissue in eels at lower temperature. The mean residence time( MRT0- ∞) and the half-life of drug( t1 /2z)in the three respective groups were( 31. 503 ± 7. 117),( 22. 881 ± 4. 940) and( 22. 134 ± 6. 204) h,and( 21. 243 ± 5. 166),( 14. 994 ± 4. 293) and( 14.656 ± 5. 061) h. These parameters showed that the elimination rate of florfenicol in eels at 24℃ or 28℃ was more quickly than that at 20℃. The areas under the concentration- time curve( AUC0- ∞) were( 235. 580 ± 62. 013),( 271. 983 ± 75. 023) and( 353. 192 ± 92. 491) μg·h/m L,respectively. It indicated that the relative bioavailability of florfenicol was higher significantly in eels at higher temperature. The present study provides a comparative understanding of florfenicol deposition kinetics following oral administration at a single dose of 30 mg / kg body weight to Japanese eel (Anguilla japonica) at the temperature of 20 ° C, 24 ° C and 28 ° C, respectively. Approximate 0. 3 mL of blood sample from each eel in these three groups were collected in a row at the time of 0.5,1,2,4,8,12,24 and 36 h after medicated-feed gavage. The concentrations of florfenicol in The plasma concentration-time data of each eel were analyzed by non-compartmental methods based on statistical moment theory using DAS software. Pharmacokinetics parameters of different groups were tested. by one-way analysis of variance using SPSS software. It was found that most of parameters were different among selected groups (P <0.05). In the three groups at 20 ° C, 24 ° C and 28 ° C, pharmacokinetics parameters of peak plasma concentra (Cmax) were (7. 839 ± 1.125), (13.10 ± 2.334) and (18.267 ± 3.777) μg / m L, and the time to reach the Cmax (6. 500 ± 2. 070), (4. 500 ± 1. 414) and (3. 429 ± 0. 926) h, respectively. These suggested that eels at higher temperature absorbed more drug and more quickly. The volumes of (Vz / F) were (3. 964 ± 0. 594), (2. 466 ± 0. 672) and (1. 841 ± 0. 485) L / kg respectively.The difference deduced that more florfenicol was bound to tissue in eels at lower temperature. The mean residence time (MRT0- ∞) and the half-life of drug (t1 / 2z) in the three respective groups were (31.503 ± 7.117), (22.881 ± These parameters showed that (4.139 ± 5.164) and (14.124 ± 4.293) and the elimination rate of florfenicol in eels at 24 ° C or 28 ° C was more quickly than that at 20 ° C. The areas under the concentration-time curve (AUC0- ∞) were (235.80 ± 62. 013), (271. 983 ± 75. 023) and (35 3. 192 ± 92. 491) μg · h / m L, respectively.It indicated that the relative bioavailability of florfenicol was higher significantly in eels at higher temperature.