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目的利用免疫损伤结合高脂饲料方法建立兔实验性动脉粥样硬化(AS)斑块形成模型,探讨影响其斑块形成的相关因素。方法日本大耳白兔30只。免疫损伤和高脂饲料组兔经耳缘静脉注射牛血清白蛋白(BSA)生理盐水溶液(250mg/kg)并喂食高脂饲料,7 d后再次经耳缘静脉注射同等剂量的BSA。另设单纯喂养高脂饲料的AS模型组和普通饲料喂食的正常兔组,72 d后取血测定动物的血脂指标、炎症因子、血管活性物质、血小板聚集黏附及血管内皮功能等指标,分析上述测定指标和兔主动脉斑块形成及病理变化的相关性。结果 72 d后,免疫损伤结合高脂饲料组和单纯高脂饲料的两组经病理观察主动脉均有斑块形成,其前者的血脂水平、血清炎症因子白介素-6(IL-6)、白介素-8(IL-8)、高敏C-反应蛋白(hs-CRP)含量均比单纯高脂饲料组显著增高(P<0.05,P<0.01)。经Pearson相关系数统计处理,上述指标和免疫损伤加高脂饲料致斑块的形成具有正相关性,和HDL-C(r=-0.58)、NO(r=-0.26)等指标具有负相关性。结论免疫损伤结合高脂饲料导致兔AS斑块形成和血脂水平、炎症因子、血小板聚集及血管活性物质等经典指标的异常有显著相关性,具有评价价值。
Objective To establish an experimental model of atherosclerotic plaque in rabbits by immunosuppression combined with high-fat diet, and to explore the related factors that influence the plaque formation. Methods Japanese white rabbits 30. Immunostaining and high-fat diet rabbits were injected bovine serum albumin (BSA) saline solution (250mg / kg) into the rabbits and fed with high-fat diet. The same dose of BSA was injected into the rabbits again 7 days later. In addition, AS model group fed with high fat diet and normal rabbits fed with normal diet were set up to measure blood lipid, inflammation factor, vasoactive substances, platelet aggregation and adhesion and vascular endothelial function after 72 d, The correlation between the index and the formation of aortic plaque and pathological changes in rabbits. Results After 72 days, both the immune injury and high-fat diet group and the high-fat diet group had plaque formation by pathological observation. The blood lipid level, serum interleukin-6 (IL-6), interleukin (IL-8) and high sensitive C-reactive protein (hs-CRP) were significantly higher than those in high fat diet group (P <0.05, P <0.01). According to Pearson correlation coefficient statistics, there was a positive correlation between the above indexes and the formation of plaque with immune injury plus high fat diet and negative correlation with HDL-C (r = -0.58) and NO (r = -0.26) . Conclusion Immunosuppression combined with high-fat diet lead to the formation of rabbit AS plaque formation and serum lipid levels, inflammatory factors, platelet aggregation and vasoactive substances such as the abnormalities of the classic indicators have a significant correlation with the evaluation value.