论文部分内容阅读
目的建立全自动氨基酸分析仪测定支链氨基酸粉中牛磺酸。方法样品经上样缓冲液(pH 2.2)溶解提取,按照氨基酸分析仪设定的钠体系程序洗脱,用Na型离子交换色谱柱分离,与茚三酮反应液在135℃下反应,生成可在570 nm和440 nm检测到的蓝色物质,采用外标法定量。结果牛磺酸在4.1065~20.5326μg/mL浓度范围内有良好的线性,r~2=0.999,回收率为101.28%,RSD为2.1%,检出限为15 mg/100 g。结论该方法准确可靠,不需要剧毒衍生试剂,适用于支链氨基酸粉中牛磺酸的测定。
Objective To establish an automatic amino acid analyzer for the determination of taurine in branched-chain amino acid powder. Methods The sample was extracted by loading buffer (pH 2.2), eluted by sodium system set by amino acid analyzer, separated by Na ion exchange column and reacted with ninhydrin reaction liquid at 135 ℃ to produce Blue substances detected at 570 nm and 440 nm were quantified by external standardization. Results The concentration of taurine in the range of 4.1065 ~ 20.5326μg / mL showed a good linearity with r ~ 2 = 0.999, recovery of 101.28% and RSD of 2.1% with a detection limit of 15 mg / 100 g. Conclusion The method is accurate and reliable, does not require virulent derivative reagents, suitable for the determination of taurine in branched-chain amino acid powder.