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目的探讨人精液一氧化氮(NO)与β2-微球蛋白(β2-MG)、纤维蛋白原(FIB)和C-反应蛋白(CRP)含量的关系。方法参照WHO标准方法,进行精液常规分析。采用镀铜镉还原荧光法检测NO代谢产物硝酸盐(NO-3)。用放射免疫法检测β2-MG。用散射比浊法检测FIB。用免疫比浊法测定CRP含量。结果不育活率异常组、少精子和无精子症组的NO含量明显高于正常生育组,β2-MG、FIB和CRP含量明显低于正常生育组,两组之间存在有高度显著性差异(P<0.01),不育组NO含量与β2-MG、FIB和CRP含量呈显著性负相关(r=-0.86、r=-0.95、r=-0.43)。结论精液蛋白质含量测定有助于精子质量的评价,对男性不育症的诊治有一定的指导意义,NO对精子运动能力及蛋白质的分泌利用有抑制作用,这对不育症的机制研究有重要价值。
Objective To investigate the relationship between nitric oxide (NO) and β2-microglobulin (β2-MG), fibrinogen (FIB) and C-reactive protein (CRP) in human semen. Methods According to WHO standard methods, routine sperm analysis. The NO metabolite nitrate (NO-3) was detected by copper-cadmium reduction fluorescence method. Β2-MG was detected by radioimmunoassay. FIB was detected by nephelometry. CRP levels were determined by immunoturbidimetry. Results The levels of NO in the infertile infertility group, oligospermia and azoospermia group were significantly higher than those in normal fertility group, and the contents of β2-MG, FIB and CRP were significantly lower than those in normal fertility group. There was a highly significant difference between the two groups (P <0.01). There was a significant negative correlation between the content of NO and the content of β2-MG, FIB and CRP in infertile group (r = -0.86, r = -0.95, r = -0.43). Conclusion The determination of sperm protein content contributes to the evaluation of sperm quality, which has some guiding significance for the diagnosis and treatment of male infertility. NO can inhibit sperm motility and protein secretion, which is important for the study of the mechanism of infertility value.