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目的:探讨硫化氢(H2S)在硫酸鱼精蛋白(PS)诱导的大鼠慢性膀胱炎中的作用。方法:36只雌性SD大鼠随机分为3组(每组12只),采用膀胱灌注PS构建慢性膀胱炎症大鼠模型,Von Frey刷检测泌尿生殖区疼痛变化,尿流动力学检测膀胱功能。亚甲基蓝分光光度法测定膀胱中H2S含量,实时定量聚合酶链反应(RTPCR)筛选H2S各催化酶、P物质等m RNA表达,Western blot检测胱硫醚-γ-裂解酶(CSE)蛋白表达。结果 :模型组大鼠膀胱中,其H2S含量明显高于对照组(P<0.05)。大鼠膀胱中CSE的m RNA及蛋白水平表达升高(均P<0.05)。与对照组比较,模型组大鼠排尿间歇缩短,膀胱容量降低。而CSE抑制剂PAG干预后其排尿间歇时间延长(390.79±41.62 vs 160.18±40.10,P<0.05),膀胱容量增加(0.99±0.11 vs 0.47±0.13,P<0.05)。此外,与模型组比较,PAG组疼痛评分明显下降(P<0.05),P物质的m RNA表达也下调(P<0.05)。结论:间质性膀胱炎时,膀胱组织中由CSE催化产生的内源性H2S水平明显增加,以H2S为靶点,抑制系统和组织中H2S生成可能可以改善IC/BPS的尿频及疼痛情况。
AIM: To investigate the role of hydrogen sulfide (H2S) in protamine sulfate induced chronic cystitis in rats. Methods: Thirty-six female Sprague-Dawley rats were randomly divided into 3 groups (12 in each group). The bladder was infused with PS to establish a rat model of chronic bladder inflammation. Von Frey brush was used to detect the changes of urogenital pain. The content of H2S in the bladder was determined by methylene blue spectrophotometry. The expression of mSNA in H2S was detected by real-time quantitative polymerase chain reaction (RTPCR). The protein expression of cystathionine-γ-lyase (CSE) was detected by Western blot. Results: The content of H2S in the bladder of the model group was significantly higher than that of the control group (P <0.05). The expression of m RNA and protein of CSE in rat bladder increased (all P <0.05). Compared with the control group, the rats in the model group were shortened intermittently and their bladder capacity was decreased. However, the prolonged micturition interval prolonged after intervention with CSE inhibitor PAG (390.79 ± 41.62 vs 160.18 ± 40.10, P <0.05) and bladder volume increased (0.99 ± 0.11 vs 0.47 ± 0.13, P <0.05). In addition, compared with the model group, pain scores of PAG group decreased significantly (P <0.05), and substance P m RNA expression was also decreased (P <0.05). Conclusions: When interstitial cystitis occurs, the level of endogenous H2S catalyzed by CSE in bladder tissue increases obviously. Suppression of H2S in system and tissue by H2S may improve the frequency and pain of IC / BPS.