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目的 观察重组人碱性成纤维细胞生长因子 (rhb FGF)对缺血心肌血管结构的作用。方法 建立兔急性心肌梗死模型 ,将 rhb FGF直接四点注射入兔缺血心肌内 ,通过病理切片图像分析观察缺血心肌血管管壁及管腔变化情况。结果 rhb FGF组与生理盐水组平均管壁厚度分别为 6周 :(2 0 .70± 9.94 ) μm,(18.88± 9.6 5 ) μm,P>0 .0 5 ;12周 :(2 9.87± 12 .96 )μm,(18.13± 11.33)μm,P<0 .0 1;管壁管腔比值分别为 6周 :0 .2 5± 0 .12 ,0 .2 4± 0 .0 2 ,P>0 .0 5 ;12周 :0 .33± 0 .14 ,0 .2 4± 0 .0 9,P >0 .0 5。结论 rhb FGF促缺血心肌血管新生到一定时期 (可能小于 12周 ) ,血管数量不再增长 ,只是血管管腔增大 ,管壁增厚 ,发生血管重构。
Objective To observe the effect of recombinant human basic fibroblast growth factor (rhb FGF) on the vascular structure of ischemic myocardium. Methods A rabbit model of acute myocardial infarction was established. The rhb FGF was injected directly into the ischemic myocardium at four points. The change of vascular wall and lumen of ischemic myocardium was observed by histopathological image analysis. Results The mean wall thickness of rhb FGF group and saline group were 6 weeks (20.7 ± 9.94) μm, (18.88 ± 9.65) μm, P> 0.05, respectively. .96) μm, (18.13 ± 11.33) μm, P <0.01; and the ratio of lumen to lumen was 6 weeks: 0.25 ± 0.12, 0.24 ± 0.22, P> 0 .0 5; 12 weeks: 0 .33 ± 0 .14, 0 .2 4 ± 0 .0 9, P> 0 .0 5. Conclusions rhb FGF promotes ischemic myocardial angiogenesis to a certain period (possibly less than 12 weeks), the number of blood vessels no longer increases, but the vessel lumen enlargement, wall thickening and vessel remodeling occur.