论文部分内容阅读
目的调查了解发热伴血小板减少综合征病毒(Severe fever with thrombocytopenia syndrome virus,SFTSV)的宿主动物和传染源在动物及媒介中的分布情况,为该传染病的防治工作提供科学依据。方法采用鼠笼法在发病区野外及居民区捕鼠,计算捕获率并进行鼠种分类,取其心、肝、脾、肺、肾、脑脏器;无菌采集当地的牛、羊、猪、鸡、犬等动物血,并采集螨、蜱、蚊等节肢动物;采用荧光定量RTPCR检测鼠体脏器及螨、蜱、蚊体内SFTSV核酸;采用双抗原夹心ELISA法检测动物血清中SFTSV总抗体。结果东海县鼠类主要有黑线姬鼠、大仓鼠、小仓鼠、臭鼩鼱、褐家鼠、小家鼠6种;野鼠捕获率较高为13.47%,主要鼠种为黑线姬鼠;家鼠捕获率相对较低为6.98%,主要鼠种为小家鼠、褐家鼠;各种鼠均有不同程度感染SFTSV,野鼠的心、肝、脾、肺、肾、脑脏器感染SFTSV构成比肝脏最高为26.32%(5/19)>心、肺的21.05%(4/19)>肾、脾、脑的10.53%(2/19);室内鼠仅有心、脑脏器检出SFTSV核酸阳性,且为单一脏器感染;牛、鸡SFTSV的总感染率分别为4.55%和1.54%;节肢动物中的小盾纤恙螨及毒棘厉螨也携带SFTSV,而蜱和蚊未检出SFTSV核酸。结论鼠类、牛、鸡及节肢动物中的小盾纤恙螨及毒棘厉螨可能是该地SFTSV的宿主动物或传播媒介。
Objective To investigate the distribution of host animals and their infectious agents in animals and their media with Severe Fever and Thrombocytopenia Syndrome Virus (SFTSV), and to provide a scientific basis for the prevention and control of this infectious disease. Methods The squirrel cage method was used to catch mice in the field and residential areas in the incidence area. The capture rate was calculated and the mouse species were classified. The heart, liver, spleen, lung, kidney and brain were collected. , Chickens, dogs and other animals, collected arthropods such as mites, ticks and mosquitoes; detected SFTSV nucleic acid in the organs and mites, ticks and mosquitoes by fluorescent quantitative RTPCR; detected the total SFTSV in serum by double antigen sandwich ELISA antibody. Results There were 6 species of Apodemus agrarius, Hamster, Hamster, Siberian Hamster, Rattus norvegicus and Mus musculus in Donghai County. The capture rate of wild rats was 13.47%, and the main species was Apodemus agrarius. The capture rate of domestic rats was relatively low at 6.98%. The main mouse species were Mus musculus and Rattus norvegicus. All mice were infected with SFTSV to varying degrees, and heart, liver, spleen, lung, kidney and brain were infected by SFTSV (2/19)> 21.3% (4/19) of heart and lung,> 10.53% (2/19) of kidney, spleen and brain. Only SFTSV The positive rate of SFTSV in cattle and chickens was 4.55% and 1.54%, respectively. SFRSV was also carried in the arthropods, but not in ticks and mosquitoes SFTSV nucleic acid. Conclusion The small shield chigger mites and the spines of Rattus norvegicus in mice, cattle, chickens and arthropods may be the host animals or vectors of SFTSV in this area.