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本文采用Ames试验,姊妹染色单体互换试验,小鼠微核试验,小鼠精子畸形试验对二氯甲烷(CH_2Cl_2)的致突变性进行研究。实验结果表明、其在空气中浓度为48000-120000mg/M~3时对试验菌株TA98,TA100具有诱变作用,在培养液中二氯甲烷的浓度为0.4258—42.58mg/ml时能诱发人体外周血淋巴细胞SCE频率明显增加(P<0.001),但未见二氯甲烷对小鼠骨髓嗜多染红细胞微核率,小鼠精子畸形率有明显影响。本研究结果在体外试验为阳性,体内试验为阴性可能由于体内外酶系统,和/或测试遗传学终点差异所致。
In this paper, Ames test, sister chromatid exchange experiments, mouse micronucleus test, mouse sperm deformity test dichloromethane (CH 2 Cl 2) mutagenicity. The experimental results show that when the concentration of 48000-120000mg / M ~ 3 in the air when the test strains TA98, TA100 mutagenic effect in the culture medium dichloromethane concentration of 0.4258-42.58mg / ml can induce the human body peripheral The frequency of SCE in blood lymphocytes increased significantly (P <0.001), but no significant effect of methylene chloride on micronucleus rate of mouse bone marrow polychromatic erythrocytes and mouse sperm deformity was observed. The results of this study were positive in vitro and negative in vivo may be due to endogenous and in vitro enzyme systems, and / or end-of-test genetic differences.