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该衣原体组抗原进行补体结合试验常用作衣原体属(鸟疫——鹦鹉热和性病淋巴肉芽肿或腹股沟淋巴肉芽肿病)的病例血清学诊断。这种补体结合试验在病人发病期间,抗体滴度高于32的病例有诊断意义。制备组抗原方法:将Hela—229细胞(ATCC-CCI-2.1)放培养于有10%的胎牛血清BHK-21培养基里,每个星期换液时减少血清含量。衣原体株:Jones在1950年从鸡胚卵黄囊分离的沙眼衣原体MRC-1株(ATCC-VRI)接种于含细胞松弛素B的Hela-229细胞培养瓶中。已感染细胞:用0.2g/l EDTA钠溶液9ml和25%的胰蛋白酶液1ml的混合液消化使细胞脱落,将细胞悬液稀释成每毫升含细胞2×10~6个。0.2ml中感染单位为10~9,即每个细胞为10~2感染单位。这种悬液再分装于直径为16mm的平底试管里,置5%CO_2培养箱,pH7.2培养,36℃ 4000g/小时速度离心,再把沉在试管
The Chlamydia antigen is subjected to complement fixation assays for serological diagnosis of cases of Chlamydia (avian-parrot heat and venereal lymphatic granuloma or inguinal lympocytoma). This complement fixation test during the patient’s onset, antibody titers higher than 32 cases of diagnostic significance. Preparation of Group Antigens Methods: Hela-229 cells (ATCC-CCI-2.1) were cultured in 10% fetal bovine serum BHK-21 medium and serum was reduced every week when fluids changed. Chlamydia Strains: Chlamydia trachomatis MRC-1 strain (ATCC-VRI) isolated from chick embryo yolk sacs in Jones in Jones was inoculated into cytochalasin B-containing Hela-229 cell culture flasks. Infected cells: The cells were detached by a mixture of 9 ml of 0.2 g / l sodium EDTA solution and 1 ml of a 25% trypsin solution, and the cell suspension was diluted to 2 x 10 6 cells / ml. 0.2ml infection unit is 10 to 9, that is, each cell is 10 to 2 infected units. This suspension is subdivided into flat-bottomed test tubes with a diameter of 16 mm, set in a 5% CO 2 incubator, cultured at pH 7.2, centrifuged at 3600C at a rate of 4000 g / hour, and then centrifuged in a test tube