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从生长在俄勒冈州的五个天然种群中采集种子,用来检验赤杨的各个半同胞家系的生物量产量和固氮能力。种子经萌发后,将幼苗放在温室中培育,对氮素营养作两种处理,一部分用本地根瘤接种,另一部分不接种。在接种前的第五周,各个家系和种源之间在大小和重量方面几乎并无差异。而在第十四周时,用当地内生根瘤菌接种的幼苗,长得比未接种的要大10～15倍。在接种的当中,不同家系和种源之间显示了生物量产量和氮素同化能力方面的差异,这说明存在着遗传性变异,并且有可能通过选种获得遗传增益。在试验中测定,凡是立地指数为95和 65的种源子代的表现要比立地指数为100或110的为妤,所以立地指数不能作为赤杨固氮能力和生物量生产能力的唯一选种标准。
Seeds were harvested from five natural populations grown in Oregon to test biomass production and nitrogen fixation in each half-sib family of Alnus sibirica. Seed germination, the seedlings on the greenhouse cultivation, nitrogen nutrition for two treatments, part of the local root nodules vaccination, the other part of the non-vaccination. In the fifth week before inoculation, there is almost no difference in size and weight between the various families and provenances. In the fourteenth week, seedlings inoculated with indigenous endogenous Rhizobium grew 10 to 15 times longer than uninoculated. Differences in biomass production and nitrogen assimilation were shown between different lineages and provenances during inoculation, indicating the existence of genetic variation and the possibility of gaining genetic gain through selection. In the experiment, all the provenance progeny with the 95 and 65 locality showed the performance index of 100 or 110 compared with the locality index, so the in situ index can not be used as the only selection standard of the alfalfa nitrogen fixing ability and the biomass production capacity .