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目的观察五倍子降低大肠杆菌耐药性的效果。方法利用影印培养法,对耐药性消除菌落进行筛选,利用微量肉汤稀释法,对抗菌药物敏感性菌落变化进行检测,并对消除子内质粒的丢失情况进行检测。结果应用二分之一MIC五倍子提取物,进行48 h反应,在庆大霉素耐药性消除率方面,大肠杆菌E11、E22、E33、E44、E55分别为24.39%、11.66%、10.34%、5.34%、16.66%。相比于十二烷基硫酸钠处理组的2.91%,效果更为明显。对多种抗菌药物来说,耐药消除子的MIC值均有所降低。对头孢唑啉、地美环素、卡那霉素、庆大霉素来说,仅有个别菌有所恢复,其余均转变为敏感,MIC下降倍数为256倍。结论对于大肠杆菌等耐药菌来说,应用五倍子能有效降低其耐药性。
Objective To observe the effect of gallnut on reducing drug resistance in E. coli. Methods Screening of drug-resistant colonies was carried out by photocopy culture method. Micro-broth dilution method was used to detect the change of antibacterial drug-sensitive colonies, and the loss of intracellular plasmids was detected. Results Efficacy of gentamicin in terms of the elimination rate of gentamycin was 24.39%, 11.66% and 10.34% for E.coli E11, E22, E33, E44 and E55, respectively. 5.34%, 16.66%. Compared with sodium dodecyl sulfate treatment group 2.91%, the effect is more obvious. For a variety of antimicrobial agents, the resistance of MICs were reduced. For cefazolin, demeclocycline, kanamycin, gentamicin, only a few individual bacteria recovered, the rest were sensitive, MIC multiple of 256 times. Conclusion For E. coli and other resistant bacteria, the application of gall nut can effectively reduce its resistance.