论文部分内容阅读
目的观察脐带间充质干细胞(umbilical cord mesenchymal stem cells,UC-MSCs)治疗异丙基肾上腺素致心力衰竭(heart failure,HF)大鼠对心肌组织Toll样受体4(Toll-like receptor 4,TLR4)表达的影响,并初步探讨其可能作用机制。方法雄性SD大鼠60只,空白组16只不做处理正常饲养;剩余大鼠44只用异丙基肾上腺素建立心力衰竭大鼠模型,32只心力衰竭大鼠制模成功,随机分为对照组(n=16)和干细胞移植组(n=16)。在治疗前和治疗后1个月,应用流式细胞仪检测外周血单核细胞表面TLR4的表达,检测血清肿瘤坏死因子αlpha(tumor necrosis factor-α,TNF-α)水平和N末端脑利钠肽前体(N terminal pro-brain natriuretic peptide,NT-proBNP)。结果治疗后对照组、移植组的TLR4的表达、TNF-α和NT-proBNP水平均较本组治疗前显著降低(P<0.05),移植组的下降值显著高于对照组(P<0.05);TLR4表达与血清TNF-α水平呈正相关(P<0.05)。结论 UC-MSCs可显著降低TLR4和TNF-α的表达,改善心力衰竭症状。
Objective To observe the effect of umbilical cord mesenchymal stem cells (UC-MSCs) on the expression of Toll-like receptor 4 (Toll-like receptor 4) in rat heart failure induced by isoproterenol TLR4) expression, and to explore its possible mechanism. Methods Sixty male Sprague-Dawley rats were randomly divided into control group (n = 16) and untreated group (n = 16). Thirty-four rats in the control group were given isoproterenol to establish a rat model of heart failure. Group (n = 16) and stem cell transplantation group (n = 16). Before treatment and 1 month after treatment, the expression of TLR4 on peripheral blood mononuclear cells was detected by flow cytometry, and the levels of serum tumor necrosis factor-α (TNF-α) and N-terminal brain natriuretic peptide N terminal pro-brain natriuretic peptide (NT-proBNP). Results After treatment, the levels of TLR4, TNF-α and NT-proBNP in control group and transplantation group were significantly lower than those before treatment (P <0.05), and those in transplantation group were significantly lower than those in control group (P <0.05) ; TLR4 expression and serum TNF-α levels were positively correlated (P <0.05). Conclusion UC-MSCs can significantly reduce the expression of TLR4 and TNF-α and improve the symptoms of heart failure.