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目的:分析非小细胞性肺癌(NSCLC)中Runx3基因启动子区甲基化状态。方法:运用甲基化特异性PCR技术检测62例NSCLC组织和癌旁正常肺组织中Runx3基因启动子甲基化,并分析该基因启动子甲基化Runx3基因mRNA和蛋白表达的影响及其与临床特征之间的关系。结果:NSCLC组织中Runx3基因异常甲基化率(48.4%)显著高于癌旁正常肺组织中Runx3基因的异常甲基化率(17.7%,P=0.000);发生完全或者不完全甲基化的NSCLC组织或者正常肺组织中Runx3基因mRNA和蛋白表达显著降低。Runx3基因启动子区高甲基化和肿瘤分化程度及临床分期有相关性(P=0.041和0.009),而与NSCLC患者性别、年龄、有无吸烟史及肿瘤类型等临床特征无关(P=0.400,0.301,0.290和0.965)。结论:Runx3基因启动子区异常甲基化是导致Runx3基因在NSCLC中表达下调的重要因素,有望成为NSCLC早期辅助诊断的分子标志物之一。
Objective: To analyze the methylation status of Runx3 promoter in non-small cell lung cancer (NSCLC). Methods: The promoter methylation of Runx3 gene in 62 cases of NSCLC tissues and adjacent normal lung tissues was detected by methylation-specific PCR. The expression of Runx3 mRNA and protein in promoter region was analyzed Relationship between clinical features. Results: The abnormal methylation rate of Runx3 gene (48.4%) in NSCLC tissues was significantly higher than that of Runx3 gene in normal lung tissues (17.7%, P = 0.000). Complete or incomplete methylation Of NSCLC tissue or normal lung tissue Runx3 gene mRNA and protein expression was significantly reduced. There was no correlation between the hypermethylation of Runx3 promoter region and tumor differentiation and clinical stage (P = 0.041 and 0.009), but not with clinical features such as gender, age, smoking history and tumor type in NSCLC patients (P = 0.400,0.301 , 0.290 and 0.965). Conclusion: Aberrant methylation of Runx3 promoter is an important factor that leads to the down-regulation of Runx3 gene expression in NSCLC. It may be one of the molecular markers for early diagnosis of NSCLC.