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为了解迁飞种群与居留种群后代发生再猖獗的生理生化差异,探讨再猖獗的机制,比较研究了在两个水稻品种(TN1和协优963)上施用杀虫剂后褐飞虱Nilaparvata lugens(Stal)迁飞虫和本地虫后代3龄、5龄若虫和成虫体内可溶性糖和粗脂肪含量以及迁飞成虫与其后代成虫体内游离氨基酸含量的变化。结果表明:施药以及未施药处理(对照)TN1水稻品种上的迁飞后代3龄、5龄若虫和成虫体内的可溶性糖含量均显著高于本地虫。与可溶性糖含量相比,施药以及对照TN1水稻品种上的迁飞后代3龄、5龄若虫和成虫体内的粗脂肪含量均显著低于本地虫。协优963上3龄、5龄若虫体内可溶性糖含量的变化趋势与TN1上相同。对照水稻上迁飞成虫的粗脂肪含量显著高于本地种群,迁飞与本地3龄、5龄若虫间粗脂肪含量没有显著差异。杀虫剂处理后的水稻上迁飞后代5龄若虫和成虫体内粗脂肪含量显著高于本地虫。方差分析结果也显示,可溶性糖和粗脂肪含量的变化在虫源和杀虫剂,虫源和杀虫剂浓度以及杀虫剂类型和浓度方面有显著交互作用。两种水稻品种上,迁飞当代成虫体内的游离氨基酸含量显著低于其后代成虫。在经3种杀虫剂处理后,TN1上施用三唑磷后成虫体内的氨基酸含量显著高于施用溴氰菊酯和吡虫啉的处理,而协优963上施用溴氰菊酯和吡虫啉显著高于施用三唑磷的处理。本研究结果对深入阐明农药诱导褐飞虱再猖獗的机制具有参考价值。
In order to understand the re-rampant physiological and biochemical differences between migratory and resident populations and explore the mechanism of re-rampancy, the brown planthopper Nilaparvata lugens (Stal) after two pesticides were applied to two rice cultivars (TN1 and Xieyou 963) Contents of soluble sugar and crude fat, 3rd instar, 5th instar nymphs and adults of migratory insects and local pests and their free amino acids content in migrating adults and their offspring. The results showed that soluble sugar content of 3rd and 5th instar nymphs and adults of migrating offspring on TN1 rice cultivars were significantly higher than those on native insects after spraying and without pesticide treatment (control). Compared with the soluble sugar content, the contents of crude fat in 3rd and 5th instar nymphs and adults of migrating offspring on TN1 were significantly lower than those of native insects. The trend of soluble sugar content in 3rd and 5th instar nymphs of Xieyou 963 was the same as that on TN1. Crude fat content of migrating adults on the control rice was significantly higher than that of the native population, and there was no significant difference in the crude fat content between the migrating fly and local 3rd and 5th instar nymphs. The crude fat content of the 5th instar nymphs and adults of migrating offspring on rice after pesticide treatment was significantly higher than that of native insects. Analysis of variance also showed that changes in soluble sugar and crude fat contents had a significant interaction between insect sources and insecticides, insect sources and insecticide concentrations, and pesticide type and concentration. On both rice cultivars, the free amino acids in migrating adults were significantly lower than those of their offspring. After three kinds of insecticide treatment, the content of amino acids in TN1 was significantly higher than that in the application of deltamethrin and imidacloprid, while the application of deltamethrin and imidacloprid in Xieyou 963 was significantly higher than Treatment with triazophos. The results of this study have reference value for further elucidating the mechanism of pesticide-induced BPH re-rampancy.