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探讨力竭性运动引起的氧化应激对肠功能的影响及运动性肠功能紊乱的原因,32只SD雄性大鼠随机分为4组:即对照组(C);运动后即刻组EX;运动后30min组(EX30);运动后60min组(EX60),测定力竭性游泳后不同时相肠组织匀浆MDA、游离巯基和ATP含量。结果:运动后肠组织MDA含量在运动后30min、60min显著性增加(P<0.01);运动后Free-SH含量在运动后30min(P<0.05)和运动后60min后(P<0.01)显著下降;运动后30min组ATP含量显著下降(P<0.01);运动后30min,Ca2+-ATPase活性下降(P<0.05);运动后60min,Na+-K+-AT-Pase和Ca2+-ATPase活性均显著下降(P<0.05)。结论:运动源性自由基产生增加,使肠组织中游离巯基被氧化,导致ATP含量下降,Na+-K+-ATPase和Ca2+-ATPase活性下降,可能是造成运动性肠功能紊乱的重要因素之一。
32 SD male rats were randomly divided into 4 groups: the control group (C); immediately after exercise EX group; exercise to promote oxidative stress on intestinal function and the reason of exercise-induced intestinal dysfunction, (EX30) at 30min after exercise; and EX60 at 60min after exercise. The contents of MDA, free sulfhydryl and ATP in intestinal homogenate were determined at different time after exhaustive swimming. Results: After exercise, the content of MDA in the intestine tissue increased significantly at 30min and 60min after exercise (P <0.01). The Free-SH content decreased significantly after 30min (P <0.05) and 60min after exercise (P <0.01) (P <0.01); the activity of Ca2 + -ATPase decreased 30min after exercise (P <0.05); the activity of Na + -K + -AT-Pase and Ca2 + -ATPase decreased significantly at 60min after exercise P <0.05). CONCLUSION: The increase of motor-derived free radicals causes the oxidation of free sulfhydryl groups in the intestine, which leads to the decrease of ATP content and the decrease of Na + -K + -ATPase and Ca2 + -ATPase activity, which may be one of the important factors of exercise-induced bowel dysfunction.