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目的:分析血浆中microRNA-499(miR-499)与急性心肌梗死(AMI)诊断的相关性。方法:前瞻性连续性地招募2011-10-2014-03因胸痛而就诊于无锡市第二人医院急诊科及心血管科的患者进入本研究。在患者入院即刻即采集其血浆,以RT-PCR法检测miR-499表达。另从同期来医院体检的患者中募集105名个体作为对照。以受试者工作特征曲线法分析miR-499在胸痛人群中诊断AMI的准确性,采用Spearman法分析血浆miR-499与肌钙蛋白、肌酸激酶同工酶(CK-MB)之间的相关性。结果:AMI患者外周血miR-499较健康个体和非心肌梗死性胸痛患者明显增高。AMI患者血清miR-499在胸痛发作后持续增高,胸痛发生10h后仍然无下降趋势。血清miR-499诊断AMI的曲线下面积为0.68。miR-499与肌钙蛋白呈正相关,与CK-MB不相关。结论:血清miR-499是诊断AMI的有益标志物。
Objective: To analyze the correlation between microRNA-499 (miR-499) and acute myocardial infarction (AMI) in plasma. Methods: Prospective continuous recruitment 2011-10-2014-03 Patients with chest pain who attended the emergency department and cardiovascular department of the Second People’s Hospital of Wuxi City entered the study. The plasma was collected immediately after admission and the expression of miR-499 was detected by RT-PCR. Another from the same period to the hospital medical examination in patients recruited 105 individuals as a control. The accuracy of miR-499 in the diagnosis of AMI in patients with chest pain was analyzed using the receiver operating characteristic curve method. The correlation between plasma miR-499 and troponin and creatine kinase (CK-MB) was analyzed by Spearman’s method Sex. Results: The peripheral blood miR-499 in patients with AMI was significantly higher than those in healthy individuals and non-myocardial infarction patients. Serum levels of miR-499 in patients with AMI continued to increase after the onset of chest pain and remained unchanged after 10 hours of chest pain. The area under the curve of serum miR-499 in diagnosing AMI was 0.68. miR-499 was positively correlated with troponin, but not with CK-MB. Conclusion: Serum miR-499 is a useful marker for the diagnosis of AMI.