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将人肝癌细胞株 (HHCC)细胞和转染 β 葡萄糖苷酸酶 ( βG)基因HHCC细胞体外培养 ,观察细胞形态结构 ,用流式细胞仪测定细胞周期 ,并采用裸鼠脾种植法研究转 βG基因HHCC细胞的转移特性 ;接种后 60d收集转移灶 ,常规制片 ,HE染色镜检。结果 ,转染 βG基因HHCC细胞内多见颗粒性物质 ,微绒毛及突起增多 ,溶酶体增多 ,内质网及核糖体丰富 ,糖元颗粒堆积 ,某些细胞出现变性、坏死。转染 βG基因HHCC和HHCC相比较 ,其细胞周期发生明显改变 ,S期细胞增多 (P <0 .0 5 ) ,G2 期细胞减少 (P <0 .0 5 ) ;裸鼠脾种植后 60d ,发现试验组出现广泛转移 ,在肝、肾、肠系膜淋巴结肉眼可见转移灶 ,而对照组仅在肝细胞中有个别散在的瘤细胞。试验结果表明 ,转染 βG基因可增强HHCC细胞在裸鼠体内的转移能力。
The human hepatocellular carcinoma cell line (HHCC) and the transfection of β-glucosidase (βG) gene HHCC were cultured in vitro. The morphology of the cells was observed. The cell cycle was determined by flow cytometry. Transfer characteristics of gene HHCC cells; 60d after inoculation metastasis collection, routine production, HE staining. The results showed that more particles were observed in HHCC cells transfected with βG gene, with more microvilli and protrusions, more lysosomes, abundant endoplasmic reticulum and ribosomes, accumulation of glycogen granules and degeneration and necrosis of some cells. Compared with HHCC, the expression of βG gene in HHCC significantly changed, the number of cells in S phase increased (P <0.05), the number of cells in G2 phase decreased (P <0.05) The experimental group was found to have extensive metastasis, with metastatic lesions in the liver, kidney, and mesenteric lymph nodes, while the control group had only discrete scattered tumor cells in the hepatocytes. The results showed that transfection of βG gene enhanced the ability of HHCC cells to transfer in nude mice.