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目的 研究抑制核因子 κB(NF κB)活性对糖尿病肾病 (DN)及糖尿病大鼠肾组织纤维连接蛋白 (FN)mRNA表达的作用。方法 将纯种雄性Wistar大鼠分为 :A组为正常对照组 (1 1只 ) ,B组为糖尿病无干预组 (1 1只 ) ,C组为吡咯烷二硫基甲酸酯 (PDTC ,NF κB抑制剂 )干预组 (9只 )。饲养 1 8周后 ,以电泳迁移率变动分析技术检测肾组织NF κB活性 ,透射电镜检测肾小球基底膜厚度及系膜基质密度 (系膜基质面积 /系膜面积 ) ,逆转录PCR检测FNmRNA表达 ,收集 2 4h尿测定尿白蛋白排泄率 (UAE)。结果 NF κB活性在B组大鼠肾组织 [(1 85± 0 54)× 1 0 6 ]显著高于A组 [(0 0 7± 0 1 1 )×1 0 6 ,P <0 0 1 ] ,C组 [(0 2 5± 0 2 5)× 1 0 6 ]显著低于B组 (P <0 0 1 )。B组与A组比较 ,UAE[(2 1 8±1 98)mgvs (0 41± 0 47)mg ,P <0 0 1 ]、肾小球基底膜厚度 [(531 6± 1 0 7 6)nmvs (31 2 4± 2 5 4)nm ,P<0 0 1 ]及系膜基质密度 [(56 41± 6 78)vs (33 95± 5 2 2 ) ,P <0 0 1 ]均有显著差异 ;UAE(0 56± 0 72 )mg、肾小球基底膜厚度 (31 5 8± 2 1 4)nm及系膜基质密度 (37 97± 7 37)在C组均显著低于B组 (P值均 <0 0 1 )。FNmRNA表达在B组大鼠肾组织 (0 73± 0 2 6)显著高于A组 (0 31± 0
Objective To investigate the effect of inhibiting the activity of nuclear factor κB (NF κB) on the expression of fibronectin (FN) mRNA in diabetic nephropathy (DN) and diabetic rats. Methods Pure male Wistar rats were divided into two groups: group A was normal control group (11), group B was diabetes intervention group (11), group C was pyrrolidine dithiocarbamate (PDTC, NF κB inhibitor) intervention group (n = 9). After 18 weeks of feeding, the activity of NF-kappa B in renal tissue was detected by electrophoretic mobility shift assay. The glomerular basement membrane thickness and mesangial matrix density (mesangial matrix area / mesangial area) were detected by transmission electron microscope. FN mRNA The urinary albumin excretion rate (UAE) was measured and the urinary albumin excretion rate was determined by collecting urine for 24 hours. Results NF κB activity was significantly higher in group B than in group A [(1007 ± 0 1 1) × 10 6, P 0 01) , While that in group C [(0 2 5 ± 0 2 5) × 1 0 6] was significantly lower than that in group B (P 0 01). In group B, the thickness of glomerular basement membrane was (531 6 ± 1 0 7 6) UAE [(21 8 ± 1 98) mg vs (0 41 ± 0 47) mg, P 0 01) nm 2 (31 2 4 ± 2 5 4) nm, P 0 01 and mesangial matrix density 5641 ± 6 78 vs 33 95 ± 52 2, P 0 01, respectively (0 56 ± 0 72) mg, glomerular basement membrane thickness (31 58 ± 2 1 4) nm and mesangial matrix density (37 97 ± 7 37) in group C were significantly lower than those in group B P values were <0 0 1). The expression of FN mRNA in the kidney of group B (0 73 ± 0 2 6) was significantly higher than that of group A (0 31 ± 0