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通过对四川省东、南、西、北、中5个区域、55个县(市)水稻立枯丝核菌(Rhizoctonia solani)样本进行分离、纯化和菌丝融合鉴定,发现分离得到的55株立枯丝核菌菌株除了D42菌株外,其余均属于AG-1IA群。还发现其中一些菌株同时能与多个群发生融合,具有桥梁菌群属性。通过离体叶片致病力鉴定发现,各菌株间致病力差异显著。进一步的RAPD聚类分析显示,在相似系数为0.941处,该55株菌株可聚为8类。这一结果表明,在四川特殊的生态区条件下,四川省水稻立枯丝核菌大多数菌株保持了良好的遗传一致性,但少数菌株发生了较大程度的变异。
Isolation, purification and mycelial fusion identification of Rhizoctonia solani from 55 counties (cities) in 5 regions of East, South, West, North and Central of Sichuan Province showed that 55 isolates Rhizoctonia solani strains in addition to D42 strains, the rest belong to AG-1IA group. Also found that some of these strains can be fused with multiple groups at the same time, with the properties of bridge bacteria. Pathogenicity identification by isolated leaves found that the pathogenicity of each strain was significantly different. Further RAPD cluster analysis showed that at a similarity coefficient of 0.941, the 55 strains could be clustered into eight categories. The results showed that most strains of Rhizoctonia solani maintained good genetic consistency in Sichuan Province under the special ecological zone conditions, but a few strains had a large degree of variation.