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目的:探讨人心脏微血管内皮细胞(HCMEC)在灯盏花乙素(scu)、低能量震波(CSWT)联合作用下对血管生成相关因子的影响。方法:随机将培养至5代的HCMEC细胞分为空白对照组、CSWT组、FAK抑制+CSWT组、IBTX+CSWT组、CSWT+scu高、中、低剂量组,通过RT-q PCR、western-blot对血管内皮生长因子(VEGF)、内皮一氧化氮合成酶(e NOS)表达测定。结果:CSWT组、FAK抑制+CSWT组、IBTX+CSWT组、CSWT+scu高、中、低剂量组VEGF、e NOs mRNA表达、蛋白表达明显高于空白对照组(P<0.05),其中SWT+scu高、中、低剂量组VEGF、e NOs mRNA表达、蛋白表达相比其他3组明显高,差异有统计学意义(P<0.05)。结论:灯盏花乙素联合低能量震波能有效提高VEGF、e NOS表达,保护血管内皮细胞损伤。
OBJECTIVE: To investigate the effect of HCMEC on angiogenesis-related factors in combination with scu and low-energy shock (CSWT) in human cardiac microvascular endothelial cells (HCMEC). Methods: HCMEC cells cultured to passage 5 were randomly divided into blank control group, CSWT group, FAK inhibition + CSWT group, IBTX + CSWT group and CSWT + scu high, medium and low dose groups. RT- blot was used to detect the expression of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS). Results: The expressions of VEGF and e NOs in CSWT group, FAK inhibition + CSWT group, IBTX + CSWT group and CSWT + scu high, medium and low dose groups were significantly higher than those in blank control group (P <0.05) Compared with the other three groups, the expression of VEGF and e NOs mRNA in scu high, middle and low dose groups were significantly higher (P <0.05). Conclusion: Scaffolds combined with low energy shock wave can effectively increase the expression of VEGF and e NOS and protect the vascular endothelial cells from injury.