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目的建立保健品中左卡尼汀的LC-MS/MS的测定方法。方法待测样品经超声提取、稀释后,分别采用CAPCELLPAKCR色谱柱和PCHILIC色谱柱进行分离,最后用LC-MS/MS进行检测。结果使用CAPCELLPAKCR法检测左卡尼汀能够得到更高的灵敏度,线性范围为0～200ng/mL,相关系数为r=0.9995。结论该方法灵敏度高、准确、重现性好,可以用于一些保健食品中左卡尼汀的含量检测。
Objective To establish a LC-MS / MS method for the determination of levocarnitine in health products. Methods The samples were extracted by sonication. After dilution, they were separated by CAPCELLPAKCR column and PCHILIC column, respectively, and finally detected by LC-MS / MS. Results The CAPCELLPAKCR method was used to detect levocarnitine with higher sensitivity. The linear range was 0 ~ 200ng / mL and the correlation coefficient was 0.9995. Conclusion The method is sensitive, accurate and reproducible. It can be used for the determination of levocarnitine in some health foods.