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利用π－A曲线、布儒斯特角显微镜（BAM）及CD光谱对葡萄糖氧化酶（GOD）的甲醇溶液铺展在CdCl2水溶液和纯水表面上形成的单分子膜进行了研究，以了解GOD分子的自组装和折叠情况．发现甲醇或者CdCl2都能使GOD分子更加流水，易形成单分子膜．铺展在CdCl2溶液上比铺展在纯水上更加能使GOD的单分子膜排列紧密．在表面压较高时，能观察到形成了第二层膜，并且使GOD分子的构象从β－折叠片向α－螺旋转变。
Monolayers formed on the surface of CdCl2 aqueous solution and pure water were studied by π-A curve, Brewster’s angle microscope (BAM) and CD spectroscopy on a methanol solution of glucose oxidase (GOD) to understand the effect of GOD molecules Self-assembly and folding situation. Found that methanol or CdCl2 GOD molecules can make more water, easy to form a monolayer. Spreading on the CdCl2 solution is more capable of aligning the GOD monolayer closer to pure water. At higher surface pressures, it was observed that a second layer of film was formed and the conformation of the GOD molecule transitioned from the [beta] -sheet to the [alpha] -helix.