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目的:探索不同浓度乳香提取物作用于HL-60细胞后,白血病细胞株HL-60细胞VEGF mRNA表达及VEGF蛋白分泌及受体Flt-1的变化。方法:MTT法检测乳香提取物对细胞增殖的抑制作用,选择适当的药物浓度及作用时间;RT-PCR法检测乳香提取物处理前后HL-60细胞中VEGF mRNA表达水平;Western Blot法检测乳香提取物处理前后HL-60细胞VEGF分泌和受体Flt-1蛋白表达水平。结果:乳香提取物在15.0 mg/L时在体外能下调HL-60细胞VEGF mRNA的表达和VEGF蛋白的分泌并呈时间与剂量依赖性(P<0.05)。结论:乳香提取物在体外可以抑制HL-60细胞分泌VEGF,同时抑制HL-60细胞内VEGF及其受体Flt-1 mRNA及蛋白表达下降,具有潜在抑制急性早幼粒细胞白血病血管新生的作用,是一种可供选择的抗白血病药物。
OBJECTIVE: To explore the expression of VEGF mRNA and the secretion of VEGF and the change of Flt-1 receptor in leukemia HL-60 cells treated with different concentrations of Frankincense extract. Methods: MTT assay was used to detect the inhibitory effect of Boswellia extract on cell proliferation, and the appropriate drug concentration and duration of action were determined. The mRNA expression of VEGF mRNA in HL-60 cells was detected by RT-PCR and Western Blot VEGF secretion and receptor Flt-1 protein expression in HL-60 cells before and after treatment. Results: When the concentration of 15.0 mg / L, the extract of Boswellia serrata could down-regulate the expression of VEGF mRNA and the secretion of VEGF protein in HL-60 cells in a dose-dependent and time-dependent manner (P <0.05). CONCLUSION: Boswellia extract can inhibit the secretion of VEGF in HL-60 cells and inhibit the expression of VEGF and its receptor Flt-1 mRNA and protein in HL-60 cells in vitro, which may potentially inhibit the angiogenesis of acute promyelocytic leukemia , Is an alternative anti-leukemia drug.