论文部分内容阅读
背景:前期实验在钛基材表面制备了锌修饰硅酸钙陶瓷涂层,具有良好的化学稳定性及抗菌性能。目的:进一步观察锌修饰硅酸钙陶瓷涂层对骨整合的影响。方法:将锌修饰硅酸钙陶瓷涂层钛片(实验组)、硅酸钙陶瓷涂层钛片(对照组)及纯钛片(空白对照组)分别与前成骨细胞MC3T3-E1共同培养,检测细胞的黏附、增殖、钙化及Ⅰ型胶原和骨钙素表达。将上述3组材料分别置入新西兰大白兔股骨内,置入后1.5个月观察骨质增生及植入体与宿主骨整合情况。结果与结论:(1)体外实验:培养12 h,实验组黏附细胞数量多于对照组、空白对照组(P<0.05);培养14 d,实验组细胞增殖活性、钙结节形成能力强于对照组、空白对照组(P<0.05);培养21 d,3组细胞Ⅰ型胶原表达无差异,但实验组骨钙素表达高于对照组、空白对照组(P<0.05)。(2)体内实验:实验组骨质增生明显,涂层材料与骨界面直接接触,界面间可见新骨生成,纤维组织分隔较少;对照组可见少量骨质增生,空白对照组几乎不见骨质增生,且两组植入体表面小部分区域与宿主骨接触,大部分区域与周围的骨小梁之间有纤维组织分隔。(3)结果表明,锌修饰硅酸钙陶瓷涂层可促进钛种植体与宿主骨整合。
Background: In the previous experiment, a zinc modified calcium silicate ceramic coating was prepared on the surface of titanium substrate, which has good chemical stability and antibacterial properties. Objective: To further observe the effect of zinc-modified calcium silicate ceramic coating on osseointegration. Methods: The zinc-modified calcium silicate ceramic coated titanium plate (experimental group), calcium silicate ceramic coated titanium plate (control group) and the pure titanium plate (blank control group) were respectively co-cultured with pre-osteoblast MC3T3-E1 The cell adhesion, proliferation, calcification and the expression of type I collagen and osteocalcin were detected. The above three groups of materials were placed in New Zealand white rabbits femur, 1.5 months after implantation to observe bone hyperplasia and implant integration with the host bone. RESULTS AND CONCLUSION: (1) In vitro experiments: The number of adherent cells in experimental group was more than that in control group and blank control group (P <0.05) after cultured for 12 h. The cell proliferation activity and calcium nodule formation ability in experimental group were stronger than those of control group Control group and blank control group (P <0.05). There was no difference in type I collagen expression between the three groups on day 21, but the level of osteocalcin in experimental group was higher than that of control group and blank control group (P <0.05). (2) In vivo experiments: The bone hyperplasia in the experimental group was obvious, the coating material was in direct contact with the bone interface, the formation of new bone was observed in the interface, and the fibrous tissue was separated less. In the control group, a small amount of bone hyperplasia was seen, Proliferated, and a small part of the surface of the implant group contacted with the host bone. Most of the implants had fibrous tissue separation with the surrounding trabecular bone. (3) The results show that zinc-modified calcium silicate ceramic coating can promote the integration of titanium implants with the host bone.