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目的:建立麝香酮在大鼠肠灌注液中的GC-MS/MS测定方法,研究麝香酮的大鼠小肠吸收动力学特征。方法:采用GC-MS/MS测定大鼠肠循环液中的麝香酮含量;采用大鼠原位肠循环灌注法进行麝香酮吸收动力学研究。结果:麝香酮在各小肠段均有较好吸收;其吸收速度常数(Ka)和每小时吸收率(A),十二指肠>空肠(P<0.05),十二指肠>回肠(P<0.01)。麝香酮在小肠段的Ka,A和吸收半衰期T1/2分别为0.990 h-1,43.58%,0.705 h。结论:麝香酮在整个肠道内均有较好吸收,十二指肠Ka,T1/2优于空肠(P<0.05),十二指肠Ka,T1/2,A显著优于回肠(P<0.01,P<0.01,P<0.05),空肠与回肠无显著差异。
Objective: To establish a method for the determination of muscone in rat intestinal perfusate by GC-MS / MS and to study the absorption kinetics of musk ketone in rats. Methods: The contents of muscone in intestinal fluid of rats were determined by GC-MS / MS. Musk ketone absorption kinetics was studied by the method of intestinal circulation perfusion in rats. Results: Musk ketone absorbed well in all segments of small intestine. The absorption rate constant (Ka), hourly absorption rate (A), duodenum> jejunum (P <0.05), duodenum> ileum <0.01). Musk ketone in the small intestine Ka, A and absorption half-life T1 / 2 were 0.990 h-1, 43.58%, 0.705 h. Conclusions: Musk ketone has good absorption in the whole intestine. Ka and T1 / 2 of duodenum are better than that of jejunum (P <0.05). Ka and T1 / 2 of duodenum are significantly better than ileum (P < 0.01, P <0.01, P <0.05), there was no significant difference between jejunum and ileum.