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本文采用沙门氏菌回复突变试验研究了五种重庆绿茶的三种样品制备形式对四种直接和间接致突变剂体外致突变性的抑制作用。结果表明,绿茶丙酮提取物和水浸浓缩液对黄曲霉毒素B_1(AFB_1)和苯并(a)芘(B(a)P)两种间接致突变剂有显著抑制作用,抑制率达到89％以上。且剂量效应关系十分明显。同时接近饮用浓度的水浸液对AFB_1和B(a)P的致突变性抑制率亦达到70%以上。但五种绿茶样品无论丙酮提取物还是水浸液对2,7—二氨基芴和叠氮钠两种直接致突变剂的诱变性均无明显抑制作用,抑制率都在20％以下。对其可能机制进行了讨论
In this paper, the Salmonella reversion mutation test was used to study the inhibitory effects of three sample preparation forms of Chongqing green tea on the in vitro mutagenicity of four direct and indirect mutagens. The results showed that green tea acetone extract and aqueous extract concentrate had significant inhibitory effects on aflatoxin B 1 (AFB 1) and benzo(a)pyrene (B(a)P) indirect mutagens, with 89% inhibition rate. the above. And the dose-effect relationship is very obvious. At the same time close to the drinking concentration of the aqueous extract on the AFB_1 and B (a) P mutagenic inhibition rate also reached 70%. However, none of the five green tea samples, whether the acetone extract or the aqueous extract, had inhibitory effects on the mutagenicity of the 2, 7-diaminopyrene and sodium azide two direct mutagens, and the inhibition rate was below 20%. Discuss possible mechanisms