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目的:建立绞股蓝中多糖的测定方法,比较广西6种绞股蓝中多糖的含量。方法:采用苯酚-硫酸比色法,于490nm处测定含量。结果:该方法线性关系良好,平均加样回收率为98.7%,RSD=1.68%。绞股蓝中多糖的含量范围是0.96%～2.98%。结论:本法较准确,可作为绞股蓝中多糖的质量控制。广西产的几种绞股蓝中,扁果绞股蓝(Gynostemme campres-sum)中的多糖含量最高。
Objective: To establish a method for the determination of polysaccharides in Gynostemma pentaphyllum and compare the content of polysaccharides in six species of Gynostemma pentaphyllum in Guangxi. Methods: The content was determined at 490 nm using a phenol-sulfuric acid colorimetric method. Results: The method has a good linear relationship with an average recovery of 98.7% and RSD = 1.68%. The content of polysaccharide in Gynostemma pentaphyllum was 0.96%-2.98%. Conclusion: This method is more accurate and can be used as a quality control for polysaccharides in Gynostemma pentaphyllum. Among several gynostemma species produced in Guangxi, Gynostemme campres-sum has the highest polysaccharide content.