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目的探讨高脂诱导IR对apoE-/-小鼠TG代谢相关基因的影响。方法健康雄性apoE-/-小鼠,随机分为普食组(NF组,n=10)和高脂喂养组(HF组,n=10)共喂养16周。采用实时荧光定量PCR测定组织TG代谢相关基因:脂肪细胞甘油三酯酶(ATGL),激素敏感性脂肪酶(HSL),过氧化物酶体增殖物激活受体γ(PPARγ)mRNA表达,同时测定了脂肪组织ATGL蛋白水平。结果高脂喂养组小鼠空腹血糖、血浆胰岛素、胆固醇、游离脂肪酸(FFA)和TG明显升高(P均<0.01);肝组织和脂肪组织ATGL和PPARγmRNA的表达水平明显降低(P<0.01和P<0.05);而HSL mRNA表达没有明显变化。此外,高脂喂养组小鼠脂肪组织ATGL蛋白水平也明显低于普食组(P<0.01)。结论高脂喂养apoE-/-小鼠产生了明显的IR,可能与TG代谢相关基因及其蛋白水平的表达下调有关。
Objective To investigate the effect of high fat-induced IR on TG metabolism-related genes in apoE - / - mice. Methods Healthy male apoE - / - mice were randomly divided into general diet group (NF group, n = 10) and high fat diet group (HF group, n = 10) for 16 weeks. Real-time fluorescence quantitative PCR was used to detect the mRNA expression of TG metabolism related genes: ATGL, PPARγ mRNA, ATGL protein levels in adipose tissue. Results The fasting blood glucose, plasma insulin, cholesterol, free fatty acid (FFA) and TG were significantly increased in high fat diet group (all P <0.01). The mRNA expression of ATGL and PPARγ in liver tissue and adipose tissue were significantly decreased (P <0.01 and P <0.05), while HSL mRNA expression did not change significantly. In addition, the ATGL protein level in adipose tissue of high-fat diet group was significantly lower than that of the normal control group (P <0.01). Conclusion ApoE - / - mice fed with high fat diet had significant IR, which may be related to the down-regulation of the gene and protein level of TG metabolism.