论文部分内容阅读
目的探究黄连对不同环境下大鼠血栓素B2(TXB2)、6-酮-前列腺素F1α(6-Keto-PGF1α)的干预作用。方法Wistar大鼠随机分为空白对照组(KB组)、高温高湿组(GG组)、常温高湿组(CG组)、低温高湿组(DG组)、高温高湿黄连给药组(GGH组)以及低温高湿黄连给药组(DGH组)。各组大鼠在相应温度和湿度条件下生长45天,GGH组、DGH组按1 m L/100 g给予黄连水煎剂15天后,取材并检测大鼠全血黏度、血浆黏度、TXB2、6-Keto-PGF1α水平,实验数据用SPSS17.0软件统计分析。结果 (1)各组大鼠全血黏度:高切变率下由低到高依次为:GG组、KB组、DG组、GGH组、CG组、DGH组;低切变率下由低到高依次为:KB组、GGH组、DG组、CG组、GG组、DGH组。高温高湿、低温高湿、常温高湿条件下大鼠的全血黏度与KB组比较差异无统计学意义(P<0.05),GGH组全血低切、高切变率下的黏度与KB组比较差异无统计学意义(P>0.05)。(2)各组高切下大鼠血浆黏度由低到高依次为:KB组、GGH组、DG组、DGH组、CG组、GG组。GG组、CG组、DG组、DGH组与KB组比较,血浆黏度有差异有统计学意义(P<0.01),且GGH组与GG组有差异统计学意义(P<0.01),与KB组比较差异无统计学意义;DGH组与DG组比较差异有统计学意义(P<0.01)。(3)各组大鼠血浆TXB2水平由低到高依次为GGH组、KB组、CG组、GG组、DG组、DGH组,其中GG组、CG组、DG组、DGH组与KB组比较差异有统计学意义(P<0.05),且GGH组与GG组比较差异有统计学意义(P<0.01),DGH组与DG组比较差异有统计学意义(P<0.05)。(4)各组大鼠6-Keto-PGF1α水平由低到高依次为GG组、DG组、DGH组、GGH组、CG组、KB组,其中GG组、DG组、DGH组与KB组比较差异有统计学意义(P<0.05),而CG组与KB组比较差异无统计学意义(P>0.05);GGH组与GG组比较差异有统计学意义(P<0.05),但DGH组与DG组比较差异无统计学意义(P>0.05)。结论 DGH组、GGH组大鼠血液黏度及血浆黏度与KB组水平的差异,验证了黄连的寒性。在高湿环境下,大鼠血液流变学异常,TXB2/6-Keto-PGF1α平衡失调,黄连通过降低DG组、GG组大鼠TXB2水平,调节大鼠体内TXB2/6-Keto-PGF1α的平衡,且在高温环境下调控作用更显著。
Objective To investigate the effects of berberine on the levels of TXB2 and 6-Keto-PGF1α in rats under different environmental conditions. Methods Wistar rats were randomly divided into blank control group (KB group), high temperature and high humidity group (GG group), high temperature and humidity group (CG group), low temperature and high humidity group (DG group) GGH group) and hypoglycemic Coptis administration group (DGH group). The rats in each group were grown for 45 days under the corresponding temperature and humidity conditions. After 15 days of administration of Coptis chinensis at 1 m L / 100 g in GGH group and DGH group, whole blood viscosity, plasma viscosity, TXB2, -Keto-PGF1α levels, experimental data using SPSS17.0 software statistical analysis. Results (1) The whole blood viscosity of rats in each group from low to high at high shear rate were as follows: GG group, KB group, DG group, GGH group, CG group, DGH group; low shear rate High order: KB group, GGH group, DG group, CG group, GG group, DGH group. The whole blood viscosity of rats under high temperature and high humidity, low temperature and high humidity and normal temperature and high humidity had no significant difference compared with KB group (P <0.05). The viscosity of low blood pressure and high shear rate of GGH group was significantly lower than that of KB There was no significant difference between the two groups (P> 0.05). (2) The plasma viscosity of rats in each group from high to low were as follows: KB group, GGH group, DG group, DGH group, CG group and GG group. There were significant differences in plasma viscosity between GG group, CG group, DG group, DGH group and KB group (P <0.01), and there was a significant difference between GGH group and GG group (P <0.01) The difference was not statistically significant; DGH group and DG group difference was statistically significant (P <0.01). (3) The levels of TXB2 in plasma from low to high were GGH group, KB group, CG group, GG group, DG group and DGH group respectively. The GG group, CG group, DG group and DGH group were compared with KB group (P <0.05). There was significant difference between GGH group and GG group (P <0.01). The difference between DGH group and DG group was statistically significant (P <0.05). (4) The levels of 6-Keto-PGF1α in each group were GG group, DG group, DGH group, GGH group, CG group and KB group from low to high respectively. The levels of 6Get, (P <0.05), but there was no significant difference between CG group and KB group (P> 0.05). The difference between GGH group and GG group was statistically significant (P <0.05) There was no significant difference between DG group (P> 0.05). Conclusions The differences of blood viscosity and plasma viscosity between DGH group and GGH group and KB group verify the coldness of Coptis chinensis. Hyperthyroidism in rats under high humidity, TXB2 / 6-Keto-PGF1α imbalance, Coptis by reducing the level of TXB2 levels in DG and GG rats, TXB2 / 6-Keto-PGF1α balance in rats , And the role of regulation in the high temperature environment is more pronounced.