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目的对2014年重庆口岸首起输入性登革热疫情进行分子流行病学调查,确定病毒的基因型别及感染来源。方法采集2014年重庆口岸首起聚集性输入登革热疑似病例血清样本,进行IgM/IgG抗体检测、核酸检测(实时荧光RTPCR)以及病毒分离;用RT-PCR方法扩增病毒分离株E基因,进行核苷酸序列测定和进化分析。结果共采集21份血清样本,其中4份样本IgM抗体阳性,阳性率为19%,IgG均阴性;9份样本为登革热病毒1型核酸阳性。分离得到4株登革病毒,4株病毒的E基因序列同源性为100%,与印度尼西亚的毒株进化关系最近,同源性为99.1%。结论输入性登革病毒与印度尼西亚的登革病毒1型亲缘关系最近,提示其传染源极有可能在印度尼西亚。
Objective To carry out a molecular epidemiological investigation of the imported dengue fever in Chongqing port in 2014 to determine the genotype of the virus and the source of the infection. Methods Serum specimens of suspected dengue fever were collected from Chongqing port in 2014 for IgM / IgG antibody detection, nucleic acid detection (real-time fluorescence RTPCR) and virus isolation. The E gene of virus isolate was amplified by RT-PCR, Nucleotide sequence determination and evolutionary analysis. Results A total of 21 serum samples were collected, of which 4 samples were positive for IgM antibody with a positive rate of 19% and negative for IgG. Nine samples were positive for dengue virus type 1 nucleic acid. Four strains of dengue virus were isolated. The sequence homology of E gene of four viruses was 100%. The homology was 99.1% with the closest evolution in Indonesia. Conclusion The most recent genetic relationship between imported dengue virus and dengue virus type 1 in Indonesia suggests that its source of infection is likely to be in Indonesia.