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为研究精氨酸代谢的关键酶精氨酸脱羧酶(Arginine Decarboxylase,ADC)和一氧化氮合酶(Nitric-oxide Synthase,NOS)mRNA在鹅等级前卵泡中的表达情况。采用高通量测序技术对鹅等级前卵泡进行转录组测序分析,并通过荧光定量PCR对结果进行验证。通过FPKM值计算分析,这2个基因在鹅等级前卵泡中均有表达,且随着卵泡的发育,NOS mRNA的表达呈现先降低后增加再降低的趋势,在初级卵泡中的表达量达到最高值,在中白卵泡中的表达量最低。ADC mRNA的表达则呈现先增加后降低再增加的趋势,在初级卵泡中的表达量最低,在小白卵泡中的表达量最高。荧光定量PCR结果与转录组测序结果基本相同。
To study the expression of mRNA of arginine decarboxylase (ADC) and nitric oxide synthase (NOS), a key enzyme in arginine metabolism, in goose preantral follicles. High-throughput sequencing was used to analyze the transcriptome of goose preantral follicles, and the results were verified by fluorescence quantitative PCR. Through the analysis of FPKM value, the two genes were expressed in the pre-goose follicles, and with the development of the follicles, the expression of NOS mRNA decreased first, then increased and then decreased, the highest expression in the primary follicles Value, the lowest expression in the white follicles. ADC mRNA expression increased first, then decreased and then increased. The expression level of ADC mRNA was the lowest in primary follicles and the highest in small follicles. Fluorescence quantitative PCR results and transcriptome sequencing results are basically the same.