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通过对现有SDS-PAGE电泳技术的样本前处理和制胶技术的改良,开发出了一种能够同时清晰鉴别7S球蛋白组分亚基(α’,α,β-Subunit)和Gly m Bd 30 K谱带的SDS-PAGE电泳快速检测新技术。利用该方法对PI603570A×0329F6杂交组合F2群体的116个样本进行了7S球蛋白组分亚基(α’,α,β-Subunit and Gly m Bd 30K)缺失的筛选,并通过回交转育技术,将7S球蛋白组分亚基的缺失性状成功导入到黑龙江主栽品种中去。新方法可以有效地加快筛选进程,降低筛选成本,对过敏原蛋白缺失大豆种质创新具有重要意义。
Through the sample pre-treatment and gel-forming technique improvement of the existing SDS-PAGE electrophoresis technology, a novel method that can clearly identify the 7S globulin subunits (α ’, α, β-Subunit) and Gly m Bd 30 K band SDS-PAGE rapid detection of new technologies. A total of 116 samples from F2 population of PI603570A × 0329F6 hybrids were screened for deletion of 7S globulin subunit (α ’, α, β-Subunit and Gly m Bd 30K) , The 7S globin protein subunit of the trait was successfully introduced into the main cultivars in Heilongjiang go. The new method can effectively speed up the screening process and reduce the screening cost, and is of great significance to the innovation of soybean germplasm lacking the allergen protein.