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本文报道分别取自妊娠10周及16周的胎儿心肌组织块,在一般啮齿动物心肌细胞培养条件下进行培养,出现了自发搏动。搏动频率分别为21～27次/分和200～290次/分。前者连续搏动30天后,人为终止培养。后者中有一细胞团块,连续搏动长达75天,自行停止。由于组织块取材厚薄及形态不同,培养后团块生长方式亦有差别。较厚的组织块生长较慢,以后纤维母细胞及心肌细胞可呈放射状生长,亦可呈索条状向外伸延;边缘较薄的组织块,心肌细胞在早期从边缘呈球形突出,然后脱落生长。较薄层组织块由于周围纤维母细胞很快长满后,心肌组织则不断加厚生长。本文认为心肌组织块培养的优点是操作简便,搏动明显并相对稳定,持续时间较长。但是提高心肌纯度和做心肌细胞计数、定量试验,尚有一定困难。
This article reports fetal heart tissue from 10 and 16 weeks of gestation, respectively, and cultured under general rodent cardiomyocyte culture conditions, spontaneous beating occurs. Beating frequency was 21 to 27 beats / min and 200 to 290 beats / min. The former after a continuous beating 30 days, the artificial termination of culture. The latter has a cell mass, continuous pulsation up to 75 days, to stop. Due to the thickness and shape of the tissue block, the growth pattern of the clumps is also different after culturing. Thicker tissue growth slower, fibroblasts and cardiomyocytes after the radial growth may also be a strip extending outward; thin edge of the tissue mass, myocardial cells from the edge in the early spherical-shaped, and then fall off Grow. Tissue of relatively thin tissue fibroblasts soon after the overgrowth, the myocardial tissue is continuously thickened growth. This article believes that the advantages of myocardial tissue culture block is simple, pulsatile and relatively stable, longer duration. However, to improve myocardial purity and cardiomyocyte count, quantitative test, there are still some difficulties.