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目的 :研究柯萨奇B病毒性心肌炎 (VMC)、扩张型心肌病 (DCM )中病毒持续感染与中药干预作用。方法 :①通过急慢性VMC动物模型 ,用定量多聚酶链反应测脾脏白细胞 (WBC)中以及心肌匀浆中的柯萨奇B病毒核糖核酸 (CBV RNA) ,观察其病毒持续状态 ,以明确病毒持续感染与DCM的意义 ;②观察苦参中抗柯萨奇B病毒的有效成分 (SFA)干预价值。结果 :在急性VMC模型中 ,10mg·kg-1·d-1、15mg·kg-1·d-1、2 0mg·kg-1·d-1SFA分别能清除CBV RNA达 6 7.5 8%、77.0 0 %及 92 .88%。在慢性 (2 70d)VMC动物模型脾脏WBC及心肌匀浆中定量检测的结果均证明有持续感染的存在。用 2 0mg·kg-1·d-1SFA治疗后 ,在心肌匀浆中CBV RNA的清除率为 92 .77% ,脾脏中CBV RNA的清除率为 89.0 4 %。结论 :DCM与病毒持续感染有关 ,SFA能清除CBV RNA ,其清除率与剂量有关 ,并见明显减少病变心肌的病理变化。
Objective: To study the effect of continuous infection of virus and traditional Chinese medicine in Coxsackie B viral myocarditis (VMC) and dilated cardiomyopathy (DCM). Methods: (1) Coxsackie B virus RNA (CBV RNA) in spleen white blood cells (WBC) and myocardial homogenate was detected by quantitative polymerase chain reaction (PCR) in acute and chronic VMC animal models and the persistence of virus was observed to confirm the virus Infection and the significance of DCM; ② observe the anti-Coxsackie virus B in the active ingredient (SFA) intervention value. Results: In acute VMC model, 10 mg · kg-1 · d-1, 15 mg · kg-1 · d-1,2 0 mg · kg-1 · d-1SFA could clear CBV RNA to 6 7.5 8% and 77.0 0% and 92 .88%. The results of quantitative detection in the spleen WBC and myocardial homogenate in the chronic (270 days) VMC animal model all demonstrated the presence of persistent infection. After treatment with 20 mg · kg-1 · d-1SFA, the clearance rate of CBV RNA in myocardial homogenate was 92.77% and the clearance rate of CBV RNA in spleen was 89.0 4%. Conclusion: DCM is associated with persistent infection of the virus. SFA can clear the CBV RNA. The clearance rate is related to the dosage, and it can obviously reduce the pathological changes of the diseased myocardium.