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目的:研究大苞雪莲总黄酮体外自由基清除能力及抗氧化活性。方法:采用体外清除1,1-二苯基-2-三硝基苯肼自由基(DPPH·),2,2-联氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二氨盐阳离子自由基(ABTS+·),超氧阴离子(O-2·),羟基自由基(·OH),一氧化氮(NO),以及测定金属螯合能力、还原力、总抗氧化能力共8种方法对大苞雪莲总黄酮的自由基清除能力及抗氧化活性进行了评价。每组实验平行3次。结果:大苞雪莲总黄酮在一定浓度下能够有效地清除DPPH·,ABTS+·,O-2·,·OH,NO,对DPPH·,ABTS+·,·OH,NO的半数清除质量浓度分别为52.52,71.71,60.38,203.95 mg·L-1,对超氧阴离子的半数清除质量浓度为271.06 mg·L-1,小于维生素C(VC,380.10 mg·L-1),半数金属螯合质量浓度为184.52 mg·L-1,并且具有一定的还原力和总抗氧化能力。结论:大苞雪莲总黄酮具有较强的体外自由基清除能力及抗氧化活性,是一种具有较高研究价值和潜力的天然抗氧化物质。
Objective: To investigate the free radical scavenging activity and antioxidative activity of total flavonoids from Ornithodalis fortunei. Methods: The effects of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH ·), 2,2-azino-bis- Acid) diammonium cation radicals (ABTS + ·), superoxide anion (O-2 ·), hydroxyl radicals (· OH), nitric oxide (NO) and the determination of metal chelating ability, Oxidation ability of a total of eight kinds of methods of total flavonoids Scopolarian free radical scavenging capacity and antioxidant activity were evaluated. Each experiment parallel 3 times. Results: The total flavonoids of Elaeagnus angustifolia could effectively remove DPPH ·, ABTS + ·, O-2 · · · OH and NO at a certain concentration, and the half-masses of DPPH ·, ABTS + · · · OH and NO were 52.52 , 71.71,60.38 and 203.95 mg · L-1, respectively. The half-value mass concentration of superoxide anion was 271.06 mg · L-1, less than that of vitamin C (380.10 mg · L-1) 184.52 mg · L-1, and has a certain reducing power and total antioxidant capacity. CONCLUSION: The total flavonoids of Elaeagnus dahurica is a natural antioxidative substance with high research value and potential. It has strong free radical scavenging activity and antioxidant activity in vitro.