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以二氯甲烷为萃取溶剂、茶碱为定量内标物 ,建立了人血浆中奥美拉唑分离测定的毛细管电泳方法。采用毛细管区带电泳分离模式 ,在CElect FS5 0 45cm× 5 0 μm未涂层石英毛细管上 ,选择 5 0mmol/L磷酸二氢钠 12 .5mmol/L硼砂为运行缓冲液 (pH 10 .10 ) ,操作电压 16kV(+)→ (- ) ,压力进样 15Psi×s ,柱上 2 0 0nm检测 ,内源性物质不干扰毛细管电泳定量分析。奥美拉唑在血浆浓度 10 0 .0～ 5 0 0 0 .0ng/ml范围内线性关系良好 (r =0 .9991,n =5 ) ,方法回收率大于 95 % ,日内日间精密度小于 9.0 % ,并成功地应用于健康志愿者单剂量静脉滴注奥美拉唑 80mg后临床血样分析和药代动力学研究
Taking dichloromethane as extraction solvent and theophylline as quantitative internal standard, a capillary electrophoresis method was established for the determination of omeprazole in human plasma. The capillary zone electrophoresis separation mode was used to select 50 mmol / L sodium dihydrogen phosphate 12.5 mmol / L borax as the running buffer (pH 10 .10) on a CElect FS5 0 45 cm × 5 0 μm uncoated quartz capillary. Operating voltage 16kV (+) → (-), pressure injection 15Psi × s, column 200nm detection, endogenous substances do not interfere with capillary electrophoresis quantitative analysis. The omeprazole had a good linearity (r = 0.9991, n = 5) in the range of plasma concentration of 100.050.00 ng / ml with the recovery of more than 95%. The intra- and inter-day precision was less than 9.0%, and successfully applied in clinical volunteers single-dose omeprazole 80mg intravenous blood samples and pharmacokinetic study