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业已证明,酶电泳是鉴别微生物基因的一种颇有价值的方法。本文报道了对不同地区恶性疟原虫6种酶变异的调查结果。疟原虫样品有冻干的和新鲜培养的两种。前者来自冈比亚,坦桑尼亚和刚果。有的分离后培养24小时,以产生滋养体和裂殖体后冻干,有的立即用-20℃的真空罐保存。采自10个国家的15株疟原虫,送检时以新鲜的或放液氮中运送。至实验室后,仍按Trager等的方法培养。作酶分析的疟原虫,先用皂素将宿主的红细胞溶解,再离心浓集。培养的原虫是在
Enzyme electrophoresis has proven to be a valuable method of identifying microbial genes. This article reports the results of a survey of six enzyme variants of Plasmodium falciparum in different regions. Plasmodium samples were lyophilized and freshly cultivated. The former came from the Gambia, Tanzania and the Congo. Some separated and cultured 24 hours to produce trophozoites and schizonts after lyophilization, and some immediately with -20 ℃ vacuum tank preservation. Fifteen strains of Plasmodium collected from 10 countries were shipped in fresh or drained nitrogen. After the lab, according to Trager et al. For the enzyme analysis of Plasmodium, the first use of saponin to dissolve the host’s red blood cells, and then concentrated by centrifugation. Protozoa cultured in the