论文部分内容阅读
目的：研究脓毒血症时大鼠心肌Ｇ蛋白的变化。方法：免疫印迹法测定大鼠心肌细胞膜Ｇｓα、Ｇｉα２和Ｇｉα３的含量，放射免疫法测定各组心肌腺苷酸环化酶的活性。结果：脓毒血症时Ｇｓα表达无明显变化。脓毒血症晚期Ｇｉα２和Ｇｉα３含量分别升高５３．５％（Ｐ＜０．０５）和６３．７％（Ｐ＜０．０１）。基础及Ｇｐｐ（ＮＨ）Ｐ刺激的腺苷酸环化酶活性在脓毒血症晚期明显抑制。结论：大鼠心肌Ｇｉ的增加参与脓毒血症时腺苷酸环化酶信号转导系统功能障碍的发生。
Objective: To study the changes of myocardial G protein in sepsis rats. Methods: The contents of Gsα, Giα2 and Giα3 in myocardial cell membrane were determined by Western blotting, and the activity of myocardial adenylate cyclase was determined by radioimmunoassay. Results: There was no significant change of Gsα expression in sepsis. The levels of Giα2 and Giα3 increased 53.5% (P <0.05) and 63.7% (P <0.01), respectively, in late sepsis. The basal and Gpp (NH) P-stimulated adenylyl cyclase activity was significantly inhibited in the late sepsis. CONCLUSION: The increase of myocardial Gi in rats is involved in the dysfunction of adenylate cyclase signaling system in sepsis.