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目的:建立测定六味地黄胶囊中丹皮酚含量的方法。方法:采用HPLC法,色谱柱为Waters ODS C18(Φ4.6 mm×150 mm,5μm),流动相为甲醇-水(45∶55),流速为1.0 ml·min-1,检测波长为274 nm。结果:丹皮酚的线性范围为5.2～15.6μg/ml(r=0.9999),丹皮酚平均回收率为99.57%、RSD为0.76%(n=6)。结论:本文建立的分析方法简便、重现性好,可用于六味地黄胶囊中丹皮酚的含量测定。
Objective: To establish a method for determination of paeonol content in Liuwei Dihuang Capsule. Methods: HPLC method was used. The chromatographic column was Waters ODS C18(Φ4.6 mm×150 mm, 5μm). The mobile phase was methanol-water(45∶55). The flow rate was 1.0 ml·min-1. The detection wavelength was 274 nm. . Results: The linear range of paeonol was 5.2 to 15.6 μg/ml (r=0.9999). The average recovery of paeonol was 99.57% and the RSD was 0.76% (n=6). Conclusion: The analytical method established in this paper is simple and reproducible. It can be used for the determination of paeonol in Liuwei Dihuang Capsule.