通过茎尖培养实现了中药莪术试管苗的快速繁殖并从试管苗基部诱导出了愈伤组织。实验表明 :以 MS培养基为基本培养基 ,附加 ZT 4.0 mg/ L + IAA 0 .5～ 1.0 m g/ L适于丛生芽的诱导与增殖 ;附加 2 ,4- D 1.0 mg/ L+ KT4.0～ 6 .0 m g/ L 适于愈伤组织的诱导 ;附加 2 ,4- D1.0～ 2 .0 mg/ L + KT0 .2～ 0 .5 mg/ L 适于愈伤组织的继代培养 ;附加 KT 4.0 m g/ L + NAA 0 .2 m g/ L适于愈伤组织的分化。 The rapid propagation of tubers of Rhizoma Zedoariae was achieved by shoot tip culture, and callus was induced from the base of the tubelets. Experiments showed that MS medium supplemented with ZT 4.0 mg/L + IAA 0.5-1.0 mg/L was suitable for the induction and proliferation of clustered shoots with additional medium supplemented with 2,4-D 1.0 mg/L+ KT4.0. ~ 6.0 mg / L is suitable for callus induction; additional 2,4-D1.0-2. 0 mg / L + KT 0.2-0.5 mg / L is suitable for the subculture of callus Additional KT 4.0 mg / L + NAA 0.2 mg / L was suitable for callus differentiation.